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pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEB3-miRFP670nano

Plasmid

#127433

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

EB3-miRFP670nano (MAPRE3 Synthetic, Human)

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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eDrEGFR

Plasmid

#184000

Purpose

Optically controlled eDrEGFR expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrEGFR (EGFR Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEB3-miRFP703

Plasmid

#79994

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

EB3 (MAPRE3 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate2-N1

Plasmid

#31879

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrGAP

Plasmid

#38175

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR Human)

Tags

Xpress

Expression

Mammalian

Mutation

contains GAP domain amino acids 1004-1271

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
anxA10-GFP

Plasmid

#107199

Purpose

Expresses human annexin A10-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

Insert

annexin A10 (ANXA10 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-AbrGAP

Plasmid

#38177

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr GAP domain (ABR Human)

Tags

Xpress

Expression

Mammalian

Mutation

ABR GAP domain including amino acids 597-859

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
eDrcKIT

Plasmid

#184001

Purpose

Optically controlled eDrcKIT expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrcKIT (KIT Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP-Bcr in pCCL-cppt178-MNDU3

Plasmid

#38156

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Bcr (BCR Human)

Use

Lentiviral

Tags

ECFP

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pB2AR-miRFP670nano

Plasmid

#127442

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

B2AR-miRFP670nano (ADRB2 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRD408

Plasmid

#178199

Purpose

Expression of H-NS from its natural promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + hns (hns Escherichia coli)

Expression

Bacterial

Promoter

hns promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP-Bcr NE/AA

Plasmid

#36418

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

breakpoint cluster region (BCR Human)

Tags

ECFP

Expression

Mammalian

Mutation

N689A/ E690A

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-Abr-HisC

Plasmid

#32509

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR (ABR Human)

Tags

HISC

Expression

Mammalian

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
Bcr DH domain NE/AA pGEX

Plasmid

#38180

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain N689A/E690A (BCR Human)

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain N689A/E690A

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pZF-AAA

Plasmid

#127465

Purpose

coding sequence for wild type zinc finger ZF-AAA (ZiF268)

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AAA (EGR1 Human)

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Bcr GAP domain R1090A/pGEX 3X

Plasmid

#36406

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Bcr GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-Abr R683A N795A

Plasmid

#36417

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

active BCR-related (ABR Human)

Tags

EGFP

Expression

Mammalian

Mutation

R683A N795A

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
EYFP-Abr in pCCL-cppt178-MNDU3

Plasmid

#38155

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr (ABR Human)

Use

Lentiviral

Tags

EYFP

Promoter

MNDU3 = LTR promoter for U3 region of myeloprolif…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-G12-AkaLuc-P2A-Proinsulin

Plasmid

#241776

Purpose

Tested on HeLa for bioluminescence, and used for production of AAV G12-Akaluc-P2A-Proinsulin for mice.

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

AkaLuc-P2A-Proinsulin

Use

AAV

Promoter

12xUAS with TATA-box minimal promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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