We narrowed to 3,103 results for: PPL

1. pGGAselect

   Plasmid

   #195714

   Purpose

   pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSPPac-hp

   Plasmid

   #48123

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   signal peptide of Pac
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. L_NLS 41kDa

   Plasmid

   #201342

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   (NLS)SV40A4-EGFP-2PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPP6

   Plasmid

   #209968

   Purpose

   Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter Dropout (sfGFP)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

   Plasmid

   #110572

   Purpose

   Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   rhaSR-PrhaBAD-stRBS-aacC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaSR-PrhaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTn7CdrA-gfp(ASV)C

   Plasmid

   #111617

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pREDTAI

    Plasmid

    #51627

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX301-ZIM3-KRAB-PYL1

    Plasmid

    #154761

    Purpose

    Expresses ZIM3 KRAB domain fused to PYL1

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. M_NLS 41kDa

    Plasmid

    #201341

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A5-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

    Plasmid

    #110559

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Diffusive 41kDa

    Plasmid

    #201335

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMG343

    Plasmid

    #176183

    Purpose

    preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2+ccdB dropout cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-KSENB-Nup133

    Plasmid

    #87340

    Purpose

    To express human Nup133 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGM1202

    Plasmid

    #69615

    Purpose

    inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge