We narrowed to 5,839 results for: pCas

1. pCAG-MLC2(CA)-IRES-GFP

   Plasmid

   #133928

   Purpose

   Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EGFP and MLC2(CA) (MYL2 Human)
   

   Expression

   Mammalian

   Mutation

   MLC2 T18D, S19D

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS SARS-CoV-2 BA.2 Spike

   Plasmid

   #185604

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

   Insert

   SARS-CoV-2 BA.2 Spike (S )
   

   Expression

   Mammalian

   Mutation

   T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   July 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCAG-Oct3/4-IRES-Neo

   Plasmid

   #13461

   Depositor

   Shinya Yamanaka

   Article

   Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

   Insert

   Oct3/4 (Pou5f1 Mouse)
   

   Expression

   Mammalian

   Mutation

   P31S

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-MCP-SRSF3-V5-mCherry

   Plasmid

   #235090

   Purpose

   SRSF3 mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-i53-bpA-EF1BFP

   Plasmid

   #111145

   Purpose

   Expression of i53 and a BFP reporter in mammalian cells

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Inserts

   i53 (mutL Synthetic)
   BFP (BFP Synthetic)
   

   Expression

   Mammalian

   Mutation

   human Ubiquitin, contains 8 point mutations

   Promoter

   CAG promoter and EF1

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. px551-mSncg-0.27kb-HA-SpCas9

   Plasmid

   #153208

   Purpose

   Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Insert

   Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Promoter

   Truncated mSncg (0.27kb)

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-FIP200 (1-640)-TSF

   Plasmid

   #203545

   Purpose

   FIP200 NTD-TwinStrep-Flag mammalian expression

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Tags

   TwinStrep Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN396-pCAGGS-TIR1-V5-2A-HygroR

   Plasmid

   #195102

   Purpose

   Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   TIR1
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-mNaChBacMut-T2A-tdTomato

   Plasmid

   #60657

   Purpose

   Expresses mNaChBacMut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   mNaChBacMut-T2A-tdTomato
   

   Expression

   Mammalian

   Mutation

   E191K

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY0738 pCAG-NLS-SscINT

    Plasmid

    #179113

    Purpose

    SscINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SscINT
    

    Use

    CRISPR

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY0739 pCAG-NLS-SacINT

    Plasmid

    #179114

    Purpose

    SacINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SacINT
    

    Use

    CRISPR

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid

    #164903

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    Y40C mutation in the Rac1 domain whcih make the s…

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid

    #100281

    Purpose

    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa)

    Promoter

    CAG promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid

    #224583

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

    Plasmid

    #148859

    Purpose

    Mammalian Expression of HsXRN1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsXRN1-sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-CAG-SpCas9-BlastR

    Plasmid

    #107190

    Purpose

    Confers constitutive expression of SpCas9

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    SpCas9 (NEWENTRY )
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Promoter

    Chicken ß-actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid

    #198551

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8.17-m+V106W
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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