Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,672 results for: pCas

Clear keywords
Showing: 1641 - 1660 of 2672 results

  1. pMCRi

    Plasmid
    #207524
    Purpose
    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Use
    Pseudomonas vector
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207524-plasmid-map-sequence-id-409593

    Enlarge

  6. AALN-BE4max

    Plasmid
    #138161
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(AALN)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 R33A + K34A + H122L + D124N; with…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138161-plasmid-map-sequence-id-272350

    Enlarge

  7. pYPQ262m

    Plasmid
    #161522
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing
    Depositor
    Yiping Qi
    Publication
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    Use
    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…
    Expression
    Plant
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161522-plasmid-map-sequence-id-325551

    Enlarge

  9. sgAAVS1(A)

    Plasmid
    #85570
    Purpose
    Expresses sgRNA targeting human AAVS1 locus
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    adeno-associated virus integration site 1 (AAVS1 Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  11. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Publication
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

    Enlarge

  13. TC1681

    Plasmid
    #164872
    Purpose
    CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects
    Depositor
    Tian Chi
    Publication
    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hAPOBEC3A fused to PspCas13b (APOBEC3A )
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human apobec Y132D
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164872-plasmid-map-sequence-id-325775

    Enlarge

  14. pRS0035

    Plasmid
    #131125
    Purpose
    Mammalian Target-AID expression vector
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1 and UGI
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131125-plasmid-map-sequence-id-257456

    Enlarge

  16. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

    Enlarge

  18. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

    Enlarge

Showing: 1641 - 1660 of 2672 results