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We narrowed to 9,094 results for: sgRNA

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Showing: 1641 - 1660 of 9094 results

  5. pSCBE3-NG

    Plasmid
    #218159
    Purpose
    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / L1111…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218159-plasmid-map-sequence-id-433199

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  6. pEPQDKN0761

    Plasmid
    #185630
    Purpose
    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Available Since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185630-plasmid-map-sequence-id-368586

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  8. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  11. pFTK086

    Plasmid
    #171358
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    171358-plasmid-map-sequence-id-351696

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  17. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68710-plasmid-map-sequence-id-123992

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  20. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid
    #124542
    Purpose
    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41 C55G
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124542-plasmid-map-sequence-id-242329

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Showing: 1641 - 1660 of 9094 results