173,176 results

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TFORF0769

Plasmid

#141692

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MSX1 (MSX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214996

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (TMEM115 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

UBC

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB5191

Plasmid

#126911

Purpose

Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

X-4 and XII-5 gRNA

Use

CRISPR

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR

Plasmid

#64927

Purpose

FRET-based biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR (EIF4EBP1 Human, Synthetic)

Tags

Cerulean and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe neo IRES-EGFP

Plasmid

#14431

Depositor

L. Miguel Martins

Insert

IRES-EGFP

Use

Retroviral

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-ccdB

Plasmid

#14153

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA (short) wt

Plasmid

#19723

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

NOTE: short splice variant Long Sp. Variant: MV…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
Lenti-TetON-Sox2

Plasmid

#110280

Purpose

Doxycycline-inducible overexpression of Sox2

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

TRE

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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