We narrowed to 7,219 results for: 11

1. Gata3-T2A-H2B-eGFP donor

   Plasmid

   #113119

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Gata3 donor region including homology arms and T2A-H2B-eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH51288

   Plasmid

   #50269

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 Plk4(Sak) D154A (Nigg HR26)

   Plasmid

   #41164

   Depositor

   Erich Nigg

   Article

   Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

   Insert

   Plk4 D154A (PLK4 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   D154A

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

   Plasmid

   #101104

   Purpose

   Encodes dCas9(N) fused to VEGFR2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-LRF

   Plasmid

   #66936

   Purpose

   Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

   Depositor

   Alvaro Monteiro

   Article

   Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV-40

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBbB8k-csg-amylase

   Plasmid

   #166859

   Purpose

   Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.

   Depositor

   Neel Joshi

   Article

   Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

   Insert

   csgBACEFG, with α-amylase fused to CsgA via flexible linker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IB-TMEM192-mGFP

   Plasmid

   #184897

   Purpose

   Stable expression of TMEM192-mGFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EcMBP165-cpGFP.PPYF.T203V.pRSET

   Plasmid

   #33373

   Purpose

   a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

   Depositor

   Loren Looger

   Article

   Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

   Insert

   Improved MBP based Maltose Biosensor
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted between AA1…

   Promoter

   T7

   Available since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-INU-FLAG-FAM134Bs

    Plasmid

    #128261

    Purpose

    Expresses FAM134Bs in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134Bs (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

    Plasmid

    #51690

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    hisG-URA3-hisG (URA3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0091

    Plasmid

    #181955

    Purpose

    Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xFOP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mVenus-PA-PKI

    Plasmid

    #65456

    Purpose

    photoactivatable PKA inhibition

    Depositor

    Klaus Hahn

    Article

    Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

    Insert

    PKI (PKIA, Pkia Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGAL4-CREB S133A

    Plasmid

    #15222

    Depositor

    Ugo Moens

    Article

    Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

    Insert

    CREB S133A (Creb1 Rat)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Serine 133 mutated to Alanine

    Promoter

    Available since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Use

    Tags

    G protein and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pENO1-iRFP-NATr

    Plasmid

    #129731

    Purpose

    Integration plasmid to overexpress iRFP in Candida albicans

    Depositor

    Robert Wheeler

    Article

    Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

    Insert

    iRFP-Candida albicans codon-optimized
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1 from Candida albicans

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. IRES-H2B-YFP-DD

    Plasmid

    #96893

    Purpose

    Reporter to monitor translation of EMCV IRES mRNA

    Depositor

    Tobias Meyer

    Article

    Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

    Insert

    IRES-H2B-Venus-DD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. piCRg Entry

    Plasmid

    #58904

    Purpose

    Cas9/gRNA Entry expression plasmid

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HSD11B1-FLAG

    Plasmid

    #24096

    Depositor

    Moses Chao

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

    Insert

    HSD11beta1 (HSD11B1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pMLS716

    Plasmid

    #188888

    Purpose

    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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