We narrowed to 2,551 results for: pcas9

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACT4)

Plasmid

#235060

Purpose

Expresses Tad-CBE(ACT4) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACT4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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ABE8r-R153del

Plasmid

#208296

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-R153del-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM47523

Plasmid

#153221

Purpose

Level 0 zCas9i vector

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

SpCas9 with introns and 2 NLS

Use

Synthetic Biology

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-LMNA-gRNA1

Plasmid

#122507

Purpose

expresses WT spCas9 and a chimeric gRNA targeting human LMNA

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

LMNA-gRNA1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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XLone-Cas9-2A-GFP

Plasmid

#170159

Purpose

Express Cas9 and GFP upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

Cas9

Use

Synthetic Biology; Piggybac

Tags

GFP

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

July 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCC2)

Plasmid

#235067

Purpose

Expresses Tad-CBE(TCC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCC1)

Plasmid

#235066

Purpose

Expresses Tad-CBE(TCC1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCC1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-2

Plasmid

#131322

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3Amax-BlastR

Plasmid

#152997

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA-UL

Plasmid

#108551

Purpose

Bacterial Target-AID UGI-LVA vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1-UGI-LVA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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NPM1_mNeonGreen_Donor

Plasmid

#178093

Purpose

HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1-mNeonGreen HDR donor

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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