We narrowed to 15,981 results for: LEA

1. pCAG-T3-SpCAS9-NG-pA

   Plasmid

   #131465

   Purpose

   Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized SpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b I-SceI (Ctermhis -MH)

   Plasmid

   #195613

   Purpose

   Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJS124

   Plasmid

   #207593

   Purpose

   GFP-BIB H20A expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-BIB H20A
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmOrange 2-Actin

   Plasmid

   #199703

   Purpose

   visualization of cleavage furrow in living mammalian mitotic cells

   Depositor

   Kenji Sugimoto

   Article

   Tanaka et al Bioimages 25: 7-19, 2017

   Insert

   human cytoplasmic action
   

   Tags

   mOrange 2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJFRC7-UAS-NPRR_dTK

   Plasmid

   #133244

   Purpose

   Fluorescent reporter for neuropeptide release imaging

   Depositor

   David Anderson

   Article

   Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

   Insert

   drosophila tachykinin-fused codon-optimized GCaMP6s
   

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMM0553

   Plasmid

   #128989

   Purpose

   LEU2 integration vector of pZF(3BS)-mRUBY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   Leu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCW57-mcGAS-LL/RK-NLS

   Plasmid

   #185457

   Purpose

   Inducible nuclear cGAS

   Depositor

   Shitao Li

   Article

   Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

   Insert

   cGAS
   

   Use

   Lentiviral

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   L157R, L159K

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCHNFI-B2

   Plasmid

   #31405

   Depositor

   Richard Gronostajski

   Article

   Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

   Insert

   Nuclear Factor IB isoform 2 (Nfib Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Not the same as reference seq. See sequence for m…

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH6Z-806

   Plasmid

   #85255

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMM0530

    Plasmid

    #128987

    Purpose

    Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    SV40NLS-ZiF268DBD-CRY2PHR
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBW-m4046865

    Plasmid

    #153930

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pL8_3xG34-DCAF8L2_X1

    Plasmid

    #191519

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG34-DCAF8L2 X1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pL5_3xG22-DCAF8L2_X1

    Plasmid

    #191516

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG22-DCAF8L2 X1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBzCas13b

    Plasmid

    #164862

    Purpose

    Inducible expression of BzCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    Bergeyella zoohelcum Cas13b
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GPKAnls

    Plasmid

    #61094

    Purpose

    GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal

    Depositor

    Steven Green

    Article

    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

    Insert

    pkac2
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMM0623

    Plasmid

    #128998

    Purpose

    pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMM0624

    Plasmid

    #128999

    Purpose

    pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMM0621

    Plasmid

    #128996

    Purpose

    pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
    

    Expression

    Yeast

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM0617

    Plasmid

    #128993

    Purpose

    loxP-KIURA3-loxP preassembled entry vector

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Δconst-GFP

    Plasmid

    #33114

    Depositor

    Greg Phillips

    Article

    O'Leary et al Mol Biol Cell. 2011 Sep 14.

    Insert

    Pcdh-γA3 (Pcdhga3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal constant region (amino…

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only