We narrowed to 24,869 results for: Spr

1. pHEE901

   Plasmid

   #91707

   Purpose

   CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-H1-gRNA

   Plasmid

   #61089

   Purpose

   CRISPR/Cas9 system gRNA cloning

   Depositor

   Shenglin Huang

   Article

   Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

   Insert

   H1 promoter; gRNA sequences
   

   Use

   CRISPR; /cas9 grna cloning vector

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMYCO1_SpdCas9_pmcDA1

   Plasmid

   #204615

   Purpose

   CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMA-SpCas9-g5

   Plasmid

   #80788

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA-SpCas9-g6

   Plasmid

   #80789

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. miniCMV eGFP hPGK BSD

   Plasmid

   #188519

   Purpose

   Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNAy

   Plasmid

   #168294

   Purpose

   Expresses gRNA targeting the yellow gene in Drosophila

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   U6.3-gRNA[y]-scaffold
   

   Expression

   Insect

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYZ173

   Plasmid

   #98410

   Purpose

   Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
   

   Use

   CRISPR

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC10

   Plasmid

   #104784

   Purpose

   pAH595-gRNA 2xplex entry cassette

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA-SpCas9-g7

    Plasmid

    #80790

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCascade(Ptr)-entry(BsaI)

    Plasmid

    #175579

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)
    

    Expression

    Bacterial

    Mutation

    NO

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas12j2

    Plasmid

    #188498

    Purpose

    Expresses FLAG tagged Cas12j2

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAT57

    Plasmid

    #204303

    Purpose

    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pADH118

    Plasmid

    #90983

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pADH137

    Plasmid

    #90986

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    C. albicans LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    

    Use

    CRISPR

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ164

    Plasmid

    #98408

    Purpose

    Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.his3 (his3 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pADH143

    Plasmid

    #90989

    Purpose

    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pADH140

    Plasmid

    #90988

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTrex-b-NLS-hSpCas9

    Plasmid

    #62543

    Purpose

    Expression of Cas9 in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    NLS-hSpCas9
    

    Use

    CRISPR; Trypanosoma cruzi expression

    Tags

    3xFLAG and NLS

    Promoter

    Ribo-HX1

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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