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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,672 results for: pCas

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Showing: 1681 - 1700 of 2672 results

  1. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid
    #208943
    Purpose
    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208943-plasmid-map-sequence-id-415668

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  2. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid
    #208947
    Purpose
    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208947-plasmid-map-sequence-id-415686

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  7. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  8. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  9. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3xFLAG and 126aa domain from HSV-1 UL12 fused to …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  10. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Publication
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109399-plasmid-map-sequence-id-211963

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  15. pCMV-PE2-p53DD

    Plasmid
    #196582
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD
    Depositor
    Ting Zhou
    Publication
    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-mp53DD
    Expression
    Mammalian
    Mutation
    Δ40-903
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196582-plasmid-map-sequence-id-386782

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  16. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

    Plasmid
    #123611
    Purpose
    CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123611-plasmid-map-sequence-id-239339

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  17. sABE v3.22_C

    Plasmid
    #209554
    Purpose
    pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA
    Depositor
    Xue Gao
    Publication
    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
    Expression
    Mammalian
    Promoter
    pCMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209554-plasmid-map-sequence-id-415477

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  18. pCMV-PE2-tagRFP-BleoR

    Plasmid
    #192508
    Purpose
    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.
    Depositor
    Gerald Schwank
    Publication
    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-tagRFP-P2A-BleoR
    Tags
    BleoR, SV40 bpNLS, and tagRFP
    Expression
    Mammalian
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    192508-plasmid-map-sequence-id-377009

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  19. pSLQ14901

    Plasmid
    #239276
    Purpose
    Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO
    Depositor
    Stanley Qi
    Publication
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PspCas13b-hGAPDH-gRNA1 (GAPDH Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239276-plasmid-map-sequence-id-474385

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  20. sgAAVS1(B)

    Plasmid
    #85571
    Purpose
    Expresses sgRNA targeting human AAVS1 locus
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    adeno-associated virus integration site 1 (AAVS1 Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1681 - 1700 of 2672 results