We narrowed to 5,839 results for: pCas

1. pCAG-Tet-on Advanced -BGH-PA

   Plasmid

   #114379

   Purpose

   Express reverse tetracycline inducible rtTA transcriptional activator

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

   Insert

   rtTA and BGH-pA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pCaMKII-EBFP-tTAn

   Plasmid

   #172124

   Purpose

   Expression of EBFP and tTA-InteinN in CaMKII positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   pCaMKII, EBFP, tTAn-InteinN
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-AMPH1-BAR-mCherry

   Plasmid

   #85130

   Purpose

   BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cmv d0 dPspCas13b-GGS-NYTHDF1

   Plasmid

   #119855

   Purpose

   Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

   Depositor

   Bryan Dickinson

   Article

   Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

   Insert

   dCas13b-GGS-NYTHDF1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-tagRFP-NLS-HA-Bxb1

   Plasmid

   #65625

   Purpose

   Vector for cotranslational expression of tagRFP-Bxb1

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Bxb1 integrase
   

   Tags

   HA, SV40 NLS, and tagRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG mFoxg1-GFP wildtype

   Plasmid

   #73048

   Purpose

   C-terminal fusion of gfp Mouse wildtype Foxg1

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   mFoxg1 (Foxg1 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-CRY2-mCherry-MYPT169

   Plasmid

   #178526

   Purpose

   Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CRY2-mCherry-MYPT169
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-GBP1-10gly-Gal4DBD

   Plasmid

   #49438

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP1-Gal4 DNA binding domain fusion protein
   

   Expression

   Mammalian

   Mutation

   C98S (to increase protein solubility), Q3D

   Promoter

   CAG

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-mTagBFP2-T2A-iCre

    Plasmid

    #113837

    Purpose

    Monomeric TagBFP2 and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCre
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS D614 soluble Spike

    Plasmid

    #164652

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-Flag-hsDicer (D1709A)

    Plasmid

    #41588

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1709A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

14. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-wtSpCas9-H840A

    Plasmid

    #80453

    Purpose

    SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    SpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-RFP-miR-Scrint

    Plasmid

    #19825

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    Scrambled miRNA
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-FLEX-rc[Chronos-GFP]

    Plasmid

    #59056

    Purpose

    Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-MCP-VPR-2AmScarlet

    Plasmid

    #121964

    Purpose

    Mammalian expression, Transcription regulation module

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Cas13d-T2A-Citrine

    Plasmid

    #224565

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-Citrine
    

    Use

    CRISPR

    Tags

    T2A-Citrine

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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