We narrowed to 3,095 results for: pb
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Plasmid#158542PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
TagsV5ExpressionMammalianMutationAmino acids 1-480, C-terminal deletion, F188D, W2…PromoterCMVAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5
Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5Available SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5-CASC3 siRes 110-480 188/218-mut
Plasmid#158544PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
TagsV5ExpressionMammalianMutationAmino acids 110-480, C-terminal deletion, F188D, …PromoterCMVAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
Plasmid#217510PurposeKD Recombinase dependent conditional smGdP-10XV5 cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
TagssmGdP-10XV5ExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA
Plasmid#166693PurposeExpress dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.DepositorInsertsdCas9-VPR
3x Cnga1 promoter-targeting sgRNAs
UseCRISPRExpressionMammalianPromoterTRE and U6Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)
Plasmid#207356PurposeLentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
Plasmid#217512PurposeKD Recombinase dependent conditional smGdP-10XOllas cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
TagssmGdP-10XOllasExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
Plasmid#217511PurposeKD Recombinase dependent conditional smGdP-10XHA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
TagssmGdP-10XHAExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)
Plasmid#207357PurposeLentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP
Plasmid#178433PurposeCAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)DepositorInserttd-sfGFP
UseCre/Lox; Flp/frt, piggybacExpressionMammalianAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)
Plasmid#191166PurposeMammalian expression of a EGFP mutant N213D derived from EK0379.DepositorInsertEGFP(N213D)
ExpressionMammalianMutationc.637A>G(N213D; CCA>CCG)PromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
ExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
ExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-bpA-SV40pA-F-L-bpA-SV40pA-L-td-sfGFP
Plasmid#178432PurposeCAG tdGFP FLP/FRT cre/lox "AND" reporter piggyBac transposon (bpA SV40pA stop)DepositorInserttd-sfGFP
UseCre/Lox; Flp/frt, piggybacExpressionMammalianAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
TagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PBK-PRT100AH
Plasmid#44739DepositorTypeEmpty backboneAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
PBK-PRT100AH
Plasmid#44739DepositorTypeEmpty backboneAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
TagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF
Plasmid#236177PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factorDepositorInsertsTagsmTagBFP2ExpressionMammalianPromoterCMV and four ZNF35/IKZF1 sitesAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF
Plasmid#236175PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factorDepositorInsertsTagsmTagBFP2ExpressionMammalianPromoterCMV and four ZNF35/ZNF250 sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only