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pT2/shFYN1/GFP4_Seq_B

Plasmid

#206135

Purpose

Knockdown of FYN

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN shRNA (Fyn Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shFYN1/GFP4_Seq_A

Plasmid

#206134

Purpose

Knockdown of FYN

Depositor

Maria Castro

Article

Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

Insert

FYN shRNA (Fyn Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-MDmut-Gata2

Plasmid

#193091

Purpose

constitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

MDmut-Gata2

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRS193

Plasmid

#197143

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS138

Plasmid

#197142

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS140

Plasmid

#197141

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS191

Plasmid

#197140

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

EF1a

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-146

Plasmid

#197136

Purpose

CloneSelect Indel reporter encoding BC-del1 barcode

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mCsnk2b - 2

Plasmid

#198506

Purpose

lentiviral stable expression of mCsnk2b gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mCsnk2b (Csnk2b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Expression

Yeast

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1884

Plasmid

#154332

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1948

Plasmid

#154340

Purpose

Enhancer reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP

Plasmid

#108486

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by CenpB 1-166

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-vsv-Mis12-dCEN-Incenp-EGFP

Plasmid

#108478

Purpose

expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1408

Plasmid

#99032

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shIl22ra2-1928

Plasmid

#99033

Purpose

Expresses a shRNA targeting the rat Il22ra2 gene

Depositor

Amy Lasek

Article

Franklin et al Psychopharmacology (Berl). 2015 Jul;232(

Insert

Il22ra2 (Il22ra2 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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KCC4_HUMAN_D0

Plasmid

#79744

Purpose

This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC4 (SLC12A7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KS6A1_HUMAN_D1

Plasmid

#79720

Purpose

This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KS6A1 (RPS6KA1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK10_HUMAN_D0

Plasmid

#79722

Purpose

This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK10 (STK10 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only