We narrowed to 23,424 results for: cas9

1. pCBC-DT3T4

   Plasmid

   #50592

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-1tplus, U6-26p, T4
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDestTol2pA2-U6:gRNA

   Plasmid

   #63157

   Purpose

   Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAf-CRISPR-yA

   Plasmid

   #191015

   Purpose

   CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

   Insert

   yA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Aspergillus flavus U6

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-sRGN3.1-BlastR

   Plasmid

   #220964

   Purpose

   Expresses human codon-optimized sRGN3.1

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   sRGN3.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-PtH2B

   Plasmid

   #133390

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   No promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. BII-CPspaH2B

   Plasmid

   #133385

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-entry-puro

   Plasmid

   #85745

   Purpose

   Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestTol2CG2-U6:gRNA

   Plasmid

   #63156

   Purpose

   Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYN2_1

    Plasmid

    #184757

    Purpose

    Plasmid for genome editing by CRISPR/Cas9

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    

    Use

    Synthetic Biology

    Tags

    2xNLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    PGK1 and tRNA-Phe-HDV Ribozyme

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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