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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,131 results for: LEA

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Showing: 1721 - 1740 of 12131 results

  2. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

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  3. pYPQ141-ZmUbi-RZ-As

    Plasmid
    #86196
    Purpose
    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Insert
    AsCpf1 gRNA cloning site for ribozyme cleavage
    Use
    CRISPR
    Tags
    Hammerhead ribozyme and HDV ribozyme
    Expression
    Plant
    Promoter
    Maize ubiquitin 1
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    86196-plasmid-map-sequence-id-176061

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  7. pECIA14

    Plasmid
    #47051
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags
    Depositor
    Chris Garcia
    Article
    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Alkaline Phosphatase (human, placental), Flag, HR…
    Expression
    Insect
    Promoter
    Metallothionein (Copper-Inducible)
    Available Since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47051-plasmid-map-sequence-id-67169

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  9. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and HA
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119756-plasmid-map-sequence-id-233665

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  14. HTATIP (2OU2)

    Plasmid
    #33338
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP (KAT5 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pGEX6P1-N-His

    Plasmid
    #119755
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    10xHis and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119755-plasmid-map-sequence-id-233662

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  20. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Tags
    Met-6xHis
    Expression
    Bacterial
    Promoter
    T5 promoter
    Available Since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

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Showing: 1721 - 1740 of 12131 results