We narrowed to 12,857 results for: sequence

1. pL0_BC-sec_sig_pep

   Plasmid

   #202133

   Purpose

   Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Secretion signal peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pL0_BC-chl_sig_pep

   Plasmid

   #202131

   Purpose

   Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Chloroplast signal peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pT7-EGFP-C1-HsAGO2-F2V2_L

   Plasmid

   #146885

   Purpose

   Mammalian Expression of HsAGO2-F2V2

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsAGO2-F2V2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIneo-lambdaN-HA-HsAGO2-F2V2_F

   Plasmid

   #146287

   Purpose

   Mammalian Expression of HsAGO2-F2V2

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F2V2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCIneo-lambdaN-HA-HsAGO2-F470V_F

   Plasmid

   #146288

   Purpose

   Mammalian Expression of HsAGO2-F470V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F470V (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCIneo-lambdaN-HA-HsAGO2-F505V_F

   Plasmid

   #146289

   Purpose

   Mammalian Expression of HsAGO2-F505V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F505V (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-HsAGO2-F470V_E

   Plasmid

   #146225

   Purpose

   Insect Expression of HsAGO2-F470V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F470V (AGO2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-HsAGO2-F505V_E

   Plasmid

   #146226

   Purpose

   Insect Expression of HsAGO2-F505V

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   HsAGO2-F505V (AGO2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY227

   Plasmid

   #184148

   Purpose

   short crRNA generator with spacer LEA2 with WT repeat region

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   s-crRNA-LEA2-WT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Plux2

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENT-L3-pSnail-L5

    Plasmid

    #186365

    Purpose

    Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Snail regulatory sequence
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY232

    Plasmid

    #184149

    Purpose

    short tracrRNA generator with WT anti-repeat region

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-WT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLY171

    Plasmid

    #184145

    Purpose

    sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    sgRNA-LEA2-ex3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Plux2

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY283

    Plasmid

    #184156

    Purpose

    Reprogrammed short tracrRNA for hijacking mRNA of zntA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-zntA-1034
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENT-L3-pFOGl-L5

    Plasmid

    #186364

    Purpose

    Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Friend Of Gata regulatory sequence
    

    Use

    Protein expression

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR2_+10D+7U

    Plasmid

    #149393

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    7bp inserted upstream, and 10bp inserted downstre…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR2_IHF_Mut

    Plasmid

    #149396

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is mutated at key positions needed f…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge