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pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX305_3xFLAG_STAT1

Plasmid

#220323

Purpose

Expresses full length human STAT1 with an N-terminal 3xFLAG tag

Depositor

Jonathan Brown

Insert

STAT1 alpha (STAT1 Human)

Use

Lentiviral

Tags

3xFLAG

Promoter

PGK

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pNL [NlucP/SRE/Hygro] Vector

Plasmid

#236810

Purpose

Express SRE response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

SRE

Use

Luciferase

Tags

NanoLuc (R)

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-IDH1

Plasmid

#62906

Purpose

Expresses wildtype IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)

Tags

Flag

Expression

Mammalian

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS-SV40-EGFP

Plasmid

#46919

Purpose

Target EGFP gene that is stably integrated into HEK293 genome

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

GFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pQE-80L iLID (C530M)

Plasmid

#60408

Purpose

E. coli expression of iLID (C530M)

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

iLID C530M

Tags

His

Expression

Bacterial

Mutation

C530M

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_SETD8_WT

Plasmid

#82290

Purpose

Gateway Donor vector containing SETD8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SETD8 (KMT5A Human)

Use

Gateway entry vector

Mutation

P316R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 FL

Plasmid

#17648

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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