164,909 results

1. HA-TAZ-S89A

   Plasmid

   #32840

   Depositor

   Kunliang Guan

   Article

   Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S89A

   Promoter

   CMV

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (AAV8)

   Viral Prep

   #130909-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (#130909). In addition to the viral particles, you will also receive purified AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. CAG-driven, Flp-dependent expression of ChrimsonR-tdTomato. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Promoter

   CAG

   Tags

   tdTomato (Flp-dependent)

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

3. pUCmini-iCAP-PHP.AX

   Plasmid

   #195218

   Purpose

   AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

   Depositor

   Viviana Gradinaru

   Article

   Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

   Insert

   Synthetic construct isolate AAV-PHP.AX VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-nEF-Con/Fon-iC++-EYFP (AAV8)

   Viral Prep

   #137155-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-iC++-EYFP (#137155). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-iC++-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre and Flp-dependent)

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

5. CMV-OMM-GCaMP6f

   Plasmid

   #127874

   Purpose

   Fluorescent reporter for mitochondrial outer membrane Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Outer membrane localized GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAd5-B7-deltaE4

   Plasmid

   #122560

   Purpose

   Modified block 7, with deletion in E4

   Depositor

   Fred Bunz

   Article

   Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

   Insert

   Adenovirus 5 genomic region 30888-35938
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Adenovirus sequences 32874-35461 replaced with Sp…

   Promoter

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR_EF1a_flagLaM4-Notch-Gal4VP64

   Plasmid

   #216669

   Purpose

   Expresses anti-mCherry synNotch Gal4VP64

   Depositor

   Leonardo Morsut

   Insert

   anti-mCherry synNotch Gal4VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SpyTag-β-Lactamase-SpyCatcher

   Plasmid

   #52656

   Purpose

   Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

   Depositor

   Mark Howarth

   Article

   Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

   Insert

   TEM-1 β-Lactamase
   

   Use

   Tags

   SpyCatcher and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV.CBA.eGFP.2A.wtTau

   Plasmid

   #140424

   Purpose

   AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

   Depositor

   Bradley Hyman

   Article

   Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

   Insert

   eGFP-2A-Tau (MAPT Synthetic, Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL4.22-VEGF-HRE::dLUC

    Plasmid

    #128096

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Mutation

    Promoter

    inserted VEGF hypoxia response elements (HRE) (x5)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge