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We narrowed to 7,330 results for: Ank

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Showing: 4351 - 4400 of 7330 results

  6. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

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  7. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  8. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162686-plasmid-map-sequence-id-321468

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  9. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162687-plasmid-map-sequence-id-321457

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  21. pB-CuR-hSOD1

    Plasmid
    #232477
    Purpose
    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Promoter
    CMV-CuO
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232477-plasmid-map-sequence-id-471678

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  23. pIL6-PMEL-hbb

    Plasmid
    #239868
    Purpose
    Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239868-plasmid-map-sequence-id-477826

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  29. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

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  30. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
    TRAC-3' homology arm (TRAC Human)
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Available Since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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  36. pPMEL-hbb

    Plasmid
    #239863
    Purpose
    Template for in vitro transcription of PMEL (gp100), including native signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence including native signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239863-plasmid-map-sequence-id-477836

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  39. pB-TR-hSOD1

    Plasmid
    #232478
    Purpose
    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Promoter
    CMV-TetO
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232478-plasmid-map-sequence-id-471680

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  40. pCW-eGFP-FHA

    Plasmid
    #114129
    Purpose
    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    aa 2-160
    Promoter
    TRE promoter, Tet ON
    Available Since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114129-plasmid-map-sequence-id-220207

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Showing: 4351 - 4400 of 7330 results