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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,087 results for: 104

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Showing: 1741 - 1760 of 8087 results

  1. pBP-TagR_MCD22

    Plasmid
    #204104
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD22 weak RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204104-plasmid-map-sequence-id-413905

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  2. KBB528

    Plasmid
    #185104
    Purpose
    C-terminal truncation of Pom152 fused to GFP
    Depositor
    Ken Belanger
    Insert
    POM152
    Tags
    GFP
    Expression
    Yeast
    Mutation
    POM152(1-???)-HA-GFP
    Available Since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185104-plasmid-map-sequence-id-368165

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  3. SLAH2

    Plasmid
    #117104
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    SLAH2 (SLAH2 Mustard Weed)
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117104-plasmid-map-sequence-id-224997

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  4. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141104-plasmid-map-sequence-id-298612

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  5. V37m

    Plasmid
    #121043
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and mScarlet selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121043-plasmid-map-sequence-id-236355

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  6. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  7. pLKO.1-blast shGFP

    Plasmid
    #110419
    Purpose
    shGFP control, silence GFP gene, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA PolIII)
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110419-plasmid-map-sequence-id-227288

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  8. mCherry-Moesin-N-14

    Plasmid
    #55104
    Purpose
    Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Moesin (MSN Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  10. tet-pLKO.puro_shGFP

    Plasmid
    #110421
    Purpose
    shGFP control, silence GFP gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110421-plasmid-map-sequence-id-213312

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  11. tet-pLKO.puro_shLuc

    Plasmid
    #110422
    Purpose
    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110422-plasmid-map-sequence-id-213315

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  12. pGEX-6P-1-WT-short-UBASH3A

    Plasmid
    #192104
    Purpose
    Bacterial expression of human wild-type short-form UBASH3A with GST tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Tags
    GST
    Expression
    Bacterial and Mammalian
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192104-plasmid-map-sequence-id-379911

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  13. Protein2PAM_NmeN4CNNT.2

    Plasmid
    #250223
    Purpose
    Express Protein2PAM_NmeN4CNNT.2 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4CNNT.2
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 I1010K/F1017L/A1021I/C1023F/H1024D/I1034…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250223-plasmid-map-sequence-id-501763

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  14. Francisella tularensis enoyl reductase

    Plasmid
    #231104
    Purpose
    Expresses FabI under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    Enoyl-acyl carrier protein reductase
    Tags
    6x HIS, GST
    Expression
    Bacterial
    Available Since
    March 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231104-plasmid-map-sequence-id-465515

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  15. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid
    #110426
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 HuR
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA Pol III)
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110426-plasmid-map-sequence-id-213399

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  16. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  17. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

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  18. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    ER-SPLICSs-cFAST10
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210431-plasmid-map-sequence-id-415626

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  20. 4MYJ

    Plasmid
    #60104
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0346:MAC04G-A11:C228843
    Use
    Baculovirus expression
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Mutation
    See comments
    Promoter
    Polyhedrin
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1741 - 1760 of 8087 results