We narrowed to 7,387 results for: 11

1. pICSL70002

   Plasmid

   #50333

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMIR-D10 UTR

   Plasmid

   #19117

   Depositor

   Bob Weinberg

   Article

   Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

   Insert

   HOXD10 3' UTR (HOXD10 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

3. dTomato_LD 0_CS G_tTA-BFP

   Plasmid

   #58871

   Purpose

   MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSA10(gfcD-10xHis)

   Plasmid

   #176192

   Purpose

   gfcD protein expression

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMCSG26(gfcD)

   Plasmid

   #176198

   Purpose

   gfcD protein expression

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSA10(gfcBCD)

   Plasmid

   #176193

   Purpose

   expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcBCD
   

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry_LD 0_CS G_tTA-BFP

   Plasmid

   #58856

   Purpose

   MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUASTattb-cAMPr Y205A, R626A

   Plasmid

   #84932

   Purpose

   cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

   Depositor

   Justin Blau

   Article

   Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

   Insert

   cAMPr Y205A R626A
   

   Expression

   Insect

   Mutation

   PKA-C Y205A, PKA-R R626A

   Promoter

   5X UAS

   Available Since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101107

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

    Plasmid

    #188704

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNAs
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI-Neo-HA-MmUbc6/2774

    Plasmid

    #11482

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc6 (Ube2j2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pICH51277

    Plasmid

    #50268

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ1656

    Plasmid

    #51483

    Purpose

    miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. f2H ∂HD (272)

    Plasmid

    #24428

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

    Insert

    SULF2 (SULF2 Human)
    

    Tags

    FLAG, His, and Myc

    Expression

    Mammalian

    Mutation

    AA 418-716 deleted, 23AA N-terminal truncation

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pICSL50016

    Plasmid

    #50315

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    

    Use

    Plant expression

    Mutation

    Codon-optimised for A. thaliana. BsaI/BbsI sites …

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAcGP67A human LRP6 E3E4 (630–1244)

    Plasmid

    #132628

    Purpose

    expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (630–1244)
    

    Expression

    Insect

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAcGP67A human LRP6 E1E4 (20–1244)

    Plasmid

    #132629

    Purpose

    expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (20–1244)
    

    Expression

    Insect

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

    Plasmid

    #101105

    Purpose

    Encodes dCas9(C) (without VP64) fused to VEGFR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR1-dCas9(C)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge