We narrowed to 20,158 results for: Nov
-
Plasmid#99892PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA
Plasmid#113691PurposeU6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9
UseAAV, CRISPR, and Cre/LoxTagsNLSPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS_cAMPFIRE-L
Plasmid#182283PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEP313-pAAV-CMV-SaCas9-DIO-pA
Plasmid#113690PurposeA CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGMCtN-TSC10M1-sspB
Plasmid#49521PurposeNourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety siteDepositorPublicationInsertTSC10M1-sspB
Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pv6_MCPH1-TandemBRCT
Plasmid#250354PurposeExpresses tandem BRCT domain from MCPH1 fused to eGFP. Can be used to detect yH2AX at DNA damage sites.DepositorInsertMCPH1 tandem BRCT
TagsNLS, eGFP and biotintag, TEV cleavageExpressionMammalianPromoterCAGGSAvailable sinceFeb. 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-GFP
Plasmid#65445PurposeExpression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectorsDepositorInsertSV40-EGFP
UseLentiviralExpressionMammalianPromoterUBQ and SV40Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-mScarlet3-S2
Plasmid#246873PurposeExpression of mScarelt3-S2 fused with Lifeact. This construct is used for imaging actin filaments.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet3-S2
TagsLifeactExpressionMammalianPromoterCMVAvailable sinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceFeb. 24, 2023AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
CoV2-N-P199L
Plasmid#177949PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.DepositorAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAGM55273
Plasmid#153211PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY0750 pCAG-NLS-BceINT
Plasmid#179112PurposeBceINT expression vectorDepositorInsertpCAG-NLS-BceINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid#160736PurposeSingle-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ETV2_P2A_Hygro_Barcode
Plasmid#120436PurposeBarcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS1099: Dual-sgRNA.Design 4
Plasmid#159537PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 with two guide RNA cassettes
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-EYFP-WPRE-bGHpA
Plasmid#213971PurposeAAV expression of EYFP from pCALM1 promoterDepositorInsertpCALM1
UseAAVAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Hsp-eGFP-PGK-mCherry
Plasmid#191862PurposeHeat inducible eGFP reporter with constitutive mCherry markerDepositorInsertseGFP
mCherry
UseLentiviralExpressionMammalianPromoterHSPA7 promoter and PGK promoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA
Plasmid#113694PurposeU6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY0216 PASTEv1 PE2-P2A-Bxb1
Plasmid#179104PurposePASTEv1 PE2-P2A-Bxb1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCMV-PE2-P2A-Bxb1
ExpressionMammalianAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by