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Lv EF1a dCas9-HA 2A Blast

Plasmid

#102812

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

dCas9-HA

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A (dCas9)

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET29a-human histone 4-in-One

Plasmid

#223237

Purpose

Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

Depositor

Weixing Zhao

Inserts

His6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII

Tags

Dual StrepII, His6, TEV protease site, and VSVG

Expression

Bacterial

Mutation

A103G

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGOAL19

Plasmid

#20190

Depositor

Tanya Parish

Article

Parish et al Microbiology (Reading). 2000 Aug;146 ( P

Type

Empty backbone

Use

Marker cassette

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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JC Mad-1

Plasmid

#25626

Depositor

Peter Howley

Article

Howley et al J Virol. 1980 Dec . 36(3):878-82.

Insert

JC Virus Mad-1

Expression

Bacterial

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
SuperNova-N1

Plasmid

#131853

Purpose

Expresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning site

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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BRCA1 (1-304)

Plasmid

#12645

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

E3-Ring domain, Ub-ligase (BRCA1 Human)

Tags

His

Expression

Bacterial

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-CC3AI

Plasmid

#78909

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

CC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4

Plasmid

#29527

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

Wild Type

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pHAGE-RSV-tdTomato-2A-GCaMP6f

Plasmid

#80317

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6f

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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p4053 HA-E6AP isoform I

Plasmid

#8657

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform I (UBE3A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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