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pJFRC7-RFPnls-PQRv2-mCD8:GFP

Plasmid

#73974

Purpose

Protein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.

Depositor

Brian Chen

Article

Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

Inserts

sfGFP
RFP

Use

Tags

mCD8 and nls

Expression

Insect

Mutation

Promoter

20XUAS-HS

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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KVS1-HA

Plasmid

#113061

Purpose

Expresses worm KVS-1 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2005 Nov;8(11):1503-9. doi

Insert

K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug-V5His6_C

Plasmid

#146040

Purpose

Insect Expression of DmSmaug

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

DmSmaug (smg Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm2_A

Plasmid

#145881

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm3_A

Plasmid

#145882

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm5_A

Plasmid

#145884

Purpose

Insect Expression of DmLSm5

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm5 (CG6610 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm7_A

Plasmid

#145885

Purpose

Insect Expression of DmLSm7

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm7 (LSm7 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTSA-38

Plasmid

#17328

Depositor

Takeshi Sano

Article

Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

Insert

streptavidin

Use

Tags

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Promoter

Available since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
pICH44300

Plasmid

#50340

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH78133

Plasmid

#50292

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
M20 siamois-luciferase p01234

Plasmid

#17157

Depositor

Randall Moon

Article

Brannon et al Genes Dev. 1997 Sep 15. 11(18):2359-70.

Insert

siamois promoter (sia1.L Frog)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101114

Purpose

Encodes dCas9(N) fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMEP5-Flag Par6 WT

Plasmid

#24647

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

Par6 (Pard6a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pG418R-GMRWhite

Plasmid

#165883

Purpose

G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-MKL1 D381-506

Plasmid

#19853

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 381 to 506

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
Nabi2.242

Plasmid

#73754

Purpose

Nabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.

Depositor

Bradley Baker

Article

Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d

Insert

Nabi2.242

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pICH45244

Plasmid

#50277

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5' UTR, RbcS3B (AT5g38410, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHRCMV 14-3-3 sigma

Plasmid

#16552

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

14-3-3 sigma (SFN Human)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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