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pET28a-mCherryGGBsMTS

Plasmid

#133625

Purpose

Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherryGGBsMTS

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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TC1405

Plasmid

#164877

Purpose

BFP-preSTOP-GFP reporter

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

BFP-preSTOP-GFP

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pT7T-EcadTSMod

Plasmid

#99871

Purpose

RNA synthesis for microinjection into xenopus laevis embryo

Depositor

Marc Tramier

Article

Herbomel et al Sci Rep. 2017 Mar 22;7:45058. doi: 10.10

Insert

EcadTSMod

Use

Xenopus microinjection

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSDH52

Plasmid

#139049

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (includes STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 WT puroR

Plasmid

#46747

Purpose

Retroviral vector that expresses Flag-tagged wild type human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP2

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pWaldo-GFPd_PepTSt

Plasmid

#58333

Purpose

Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

Simon Newstead

Article

Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

Insert

PepTSt

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
LMAPCW

Plasmid

#205655

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Rabaptin-5

Plasmid

#199173

Purpose

Expresses GFP-Rabaptin-5 fusion in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

Rabaptin-5 (RABEP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Contains Tyr 149 Ser substitution; insert starts …

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_RYK

Plasmid

#187790

Purpose

MAC-tagged gene expression of human RYK

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RYK (RYK Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSDH61

Plasmid

#139033

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (no STOP codon)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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LMAPPW

Plasmid

#205657

Purpose

shRNAmir backbone for RNA interference, with Puromycin selection

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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TC1078-30

Plasmid

#164878

Purpose

30bp(mismatch17) cas13d gRNA for 1405 editing

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

gRNA for TC1405

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMB-PMr

Plasmid

#61181

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_66.11

Plasmid

#194213

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC

Plasmid

#148443

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-Malat

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKD016

Plasmid

#207932

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_STYK1

Plasmid

#187792

Purpose

MAC-tagged gene expression of human STYK1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

STYK1 (STYK1 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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