We narrowed to 2,582 results for: pCas
-
-
pGG369
Plasmid#165606PurposeVector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)DepositorInsertω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
UseCRISPR and Synthetic BiologyTags1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…ExpressionBacterialMutationRecoding of BsaI site in the AmpR cassette, two B…PromoterlacUV2 driving ω-dCas9 and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-CTNNB1-ATG
Plasmid#153429PurposeExpresses a gRNA that overlaps the startcodon of human CTNNB1DepositorAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE
Plasmid#139095PurposeExpresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dSpCas9-NG
UseCRISPR and LentiviralExpressionMammalianMutationD10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ENL)
Plasmid#69212PurposeLentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])DepositorInsertsUseCRISPR and LentiviralTagsFLAGAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-Hypa
Plasmid#218165PurposeThis plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in StreptomycesDepositorInsertsescbe3-NG-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS-mNAMPT
Plasmid#187887PurposeGal4/UAS expression of mammalian NAMPTDepositorInsertmNAMPT
Available SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti Cas9/SB100
Plasmid#155294PurposeLentiviral expression of Cas9 and SB100DepositorInsertSB100X-P2A-spCas9
ExpressionMammalianAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH13
Plasmid#125798PurposeLevel0 SpCas9, wheat codon optimizedDepositorInsertSpCas9, wheat codon optimized
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_275
Plasmid#179091Purposenuclease Cas expressionDepositorInsertCas9-NG
UseCRISPR and LentiviralAvailable SinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH32
Plasmid#125809PurposeLevel0 SpCas9-NG, human codon optimizedDepositorInsertSpCas9-NG, human codon optimized
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH52
Plasmid#128181PurposeLevel1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOStDepositorInsert2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH45
Plasmid#125810PurposeLevel0 nSpCas9-ABE7.10 base changer, human codon optimizedDepositorInsertnSpCas9-ABE7.10 base changer, human codon optimized
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCL.140
Plasmid#184995PurposeExpress Cas9 in human cellsDepositorInsertSpCas9
TagsSV40NLSExpressionMammalianPromoterTetOn-3gAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Guide-Generating Vector
Plasmid#125752PurposeGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assemblyDepositorInsertCys4-GFPdropout-SpCas9scaffold
UseSynthetic BiologyAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW194b63
Plasmid#136930PurposeDirected evolution of SpCas10DepositorInsertnpuC-gIII(C)
UseCRISPRExpressionBacterialMutationnoneAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-H11r1-2-DD-Cas9
Plasmid#85978PurposeExpresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cellsDepositorInsertDD-SpCas9
UseCRISPRTagsFKBP12 L106P DDExpressionMammalianAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only
