Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,702 results for: Gla

Showing: 321 - 360 of 3702 results

Results per page:

Sort results by:

pLJM1-zeo-ORF29

Plasmid

#200028

Purpose

Lentiviral vector for expression of KSHV ORF29

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-2xStrep-ORF68

Plasmid

#162659

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-zeo-2xStrep-UL52

Plasmid

#162663

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C415A

Plasmid

#162633

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C415 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 H452A

Plasmid

#162634

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 H452A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid H452 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C79A

Plasmid

#162635

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C79A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C79 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 K435A

Plasmid

#162636

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K435A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid K435 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 R443A

Plasmid

#162637

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R443A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 K450A/R451A

Plasmid

#162638

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K450 and R451 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 R14A

Plasmid

#162640

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R14A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid R14 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 K310A

Plasmid

#162641

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K310A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 R14A/K310A

Plasmid

#162642

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids R14 and K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 K395A/K396A

Plasmid

#162643

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K395 and K396 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL32 C128A

Plasmid

#162644

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C128A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C128 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL32 C502A

Plasmid

#162645

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C502A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C502 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL32 C308A/C309A

Plasmid

#162646

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C308A/C309A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids C308 and C309 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL32 C544A

Plasmid

#162647

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C544A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C544 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL32 H581A

Plasmid

#162648

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 H581A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid H581 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL32 C155A

Plasmid

#162649

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C155A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C155 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C52A

Plasmid

#162630

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C52A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C52 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C373A

Plasmid

#162631

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C373A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C373 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-ORF68 C191A/C192A

Plasmid

#162632

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 C191A/C192A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids C191 and C192 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-RNF126 C13,16A

Plasmid

#138645

Purpose

Bacterial expression of GST-RNF126 ZnF mutant (C13,16A) fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C13A, C16A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
UL79(E48A_L49A)-3xFLAG pCDNA4

Plasmid

#129759

Purpose

Expresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL79 (UL79 Human Cytomegalovirus (HHV-5))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glutamic acid 48 to Alanine and Leucine 4…

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ubiquitin (1-76; F4A)

Plasmid

#110757

Purpose

Bacterial expression of Ubiquitin (1-76; F4A)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

F4A

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416 Gal-RNQ1 L94A-YFP

Plasmid

#18692

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCas9-BFP

Plasmid

#78545

Purpose

Fusion protein Cas9+BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCas9-GFP

Plasmid

#78546

Purpose

Fusion protein Cas9+GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SOX D221A/E244A/K246A pACEmam1

Plasmid

#192050

Purpose

Expresses a 3X catalytic mutant of SOX (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (3X catalytic mutant)

Use

Unspecified

Mutation

3X catalytic mutant D221A/E244A/K246A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDECKO_mCherry

Plasmid

#78534

Purpose

Backbone to clone single or paired sgRNAs

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA)

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-TetOne-zeo_3xHA-TurboID-NLS

Plasmid

#200022

Purpose

For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

3xHA-TurboID-NLS

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3Gs

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-TetOneZeo-ORF18(WT)-3xFLAG

Plasmid

#129746

Purpose

Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3Gs and Tet inducible promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ubiquitin (1-76; T66V, L67N)

Plasmid

#110755

Purpose

Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

T66V, L67N

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS2142

Plasmid

#184273

Purpose

Tet-off genome insertion system

Depositor

Dominique Sanglard

Article

Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

Insert

CatTA

Expression

Yeast

Promoter

TDH3

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUE1-TSP-ORF68 R443A

Plasmid

#162652

Purpose

Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
K8.1Pr-pGL4.16

Plasmid

#131037

Purpose

Firefly Luciferase reporter for K8.1 promoter from KSHV

Depositor

Britt Glaunsinger

Article

Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

Insert

KSHV K8.1 100 bp promoter

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 321 - 360 of 3702 results

Results per page:

Sort results by: