Skip to main content
Addgene
Showing: 321 - 360 of 8720 results

  4. EF1a_MYC deltaLZ_P2A_Hygro_Barcode

    Plasmid
    #120509
    Purpose
    Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaLZ (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of leucine zipper motif
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120509-plasmid-map-sequence-id-234932

    Enlarge

  5. EF1a_MYC deltaHLH_P2A_Hygro_Barcode

    Plasmid
    #120508
    Purpose
    Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC deltaHLH (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of helix-loop-helix motif
    Promoter
    EF1a
    Available since
    Feb. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    120508-plasmid-map-sequence-id-234930

    Enlarge

  10. ZP57 Lef1

    Plasmid
    #16885
    Depositor
    Randall Moon
    Insert
    lef1 (lef1 Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  12. pEF1a-Foxd3-ER

    Plasmid
    #37270
    Depositor
    Patricia Labosky
    Insert
    Foxd3 (Foxd3 Mouse)
    Use
    Tags
    ER
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Aug. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  17. psLIB EF1alpha

    Plasmid
    #229954
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229954-plasmid-map-sequence-id-461280

    Enlarge

  18. pPtef1-aclB

    Plasmid
    #227343
    Purpose
    Overexpression of the aclB gene in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of AoaclB (AO090023000206)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227343-plasmid-map-sequence-id-458566

    Enlarge

  19. pPtef1-aclA

    Plasmid
    #227342
    Purpose
    Overexpression of the aclA gene in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of AoaclA (AO090023000205)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227342-plasmid-map-sequence-id-458619

    Enlarge

  22. pPB_EF1a_Gag-EIF4E-TM

    Plasmid
    #212658
    Purpose
    piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Piggybac
    Tags
    EIF4E (W56L, W102L, W166L)
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212658-plasmid-map-sequence-id-420239

    Enlarge

  30. pEF1Cas-LT1

    Plasmid
    #210556
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    210556-plasmid-map-sequence-id-426215

    Enlarge

  33. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

    Enlarge

Showing: 321 - 360 of 8720 results