We narrowed to 448 results for: DHFR
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Plasmid#172108PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFRDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only
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pDEST-DHFR F[3]-C (LEU2)
Plasmid#177796PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneTagsDHFR F[3]ExpressionYeastPromoterADH1Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
SaDHFR-pNIC-Bsa4
Plasmid#231039PurposeExpresses Staphylococcus aureus Dihydrofolate reductase under T7 with HIS tagDepositorInsertSaDHFR
Available SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBMN ltDHFR(DD)-YFP
Plasmid#47076PurposeecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegansDepositorInsertltDHFR(DD)
UseRetroviralTagsHA and YFPMutationamino acids mutations: R12H, N23S, G67S, V78A, E1…PromoterLTRAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryPromoterDHFR 5' UTRAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Mouse, Budding Yeast)
ExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-PP7-VP64_T2A_GFP
Plasmid#86167PurposeExpresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cellsDepositorInsertDHFR-PP7-VP64_T2A_GFP
UseCRISPR and LentiviralTagsDestabilized domain (N-terminus version) of E.col…ExpressionMammalianPromoterEF1aAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
YFP-DHFR-myc
Plasmid#118852PurposeFor fluorophore translocation assay and inducible tagging with zapalog.DepositorTypeEmpty backboneTagsDHFR, Myc, and YFPExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
TagsDHFR and mNeonExpressionMammalianPromoterChicken beta actin (CAG)Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-N (LEU2)
Plasmid#177798PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneExpressionYeastPromoterADH1Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
TagsDHFR and mCherryExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
TfR-moxGFP-DHFR
Plasmid#173012PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).DepositorInsertTfR-moxGFP-DHFR
TagsDHFR and moxGFPExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-ltDHFR(DD)
Plasmid#47080PurposeecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegansDepositorInsertYFP-ltDHFR(DD)
UseRetroviralTagsHA and YFPMutationamino acids mutations: R12H, N37D, N59D, G67S, D1…PromoterLTRAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330-NL-DHFR-SpCas9
Plasmid#124522PurposeExpresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cellsDepositorInsertNL-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianPromoterCbhAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
TagsDHFR and EGFPExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPRT::Floxed DHFR-TS*
Plasmid#100606PurposeTgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.DepositorInsertTgUPRT::Floxed TgDHFR-TS*
UseToxoplasma expressionMutationPyrimethamine resistancePromoterpTgDHFR-TSAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR-SIMvada
Plasmid#164649PurposeSIM mutant tagged with mCherry and eDHFRDepositorInsertSIMvada
UseCre/LoxExpressionMammalianPromoterCAGAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only