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Showing: 161 - 180 of 326 results

  2. CROPseq-Guide-BSD

    Plasmid
    #216123
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216123-plasmid-map-sequence-id-428727

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  3. pJRoC30_Th3

    Plasmid
    #188064
    Purpose
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Use
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 mutations, described in publication
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188064-plasmid-map-sequence-id-370132

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  4. pJRoC30_Tv9nL

    Plasmid
    #188069
    Purpose
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Use
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    79 PROSS mutations, described in publication
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188069-plasmid-map-sequence-id-370129

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  13. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    Promoter
    P-LlacO-1
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200837-plasmid-map-sequence-id-405511

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  14. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189620-plasmid-map-sequence-id-379033

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  15. pGEX-2T-CBP(1-287)

    Plasmid
    #182839
    Purpose
    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2014 Mar;141(5):1129-39. do
    Insert
    CBP1-287
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182839-plasmid-map-sequence-id-360063

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  16. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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Showing: 161 - 180 of 326 results