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We narrowed to 12,249 results for: car

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pcDNA3-TORCAR

Plasmid

#64927

Purpose

FRET-based biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR (EIF4EBP1 Synthetic, Human)

Use

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#178087

Purpose

HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

mScarlet-I mTOR signaling indicator (TOSI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-AncCAR_A

Plasmid

#168286

Purpose

Expresses AncCAR_A in E. coli from a T7 promoter

Depositor

Nicholas Harmer

Article

Thomas et al Commun Biol. 2019 Nov 22;2:429. doi: 10.

Insert

AncCAR_A

Use

Synthetic Biology

Tags

6*His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBS31-cCARLIN

Plasmid

#160928

Purpose

Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

Depositor

Fernando Camargo

Article

Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

Insert

CARLIN gRNAs and CAG-GFP-CARLIN array

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for gRNAs; CAG promoter for GFP

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR(T/A)

Plasmid

#64928

Purpose

Negative-control mutant for TORCAR biosensor.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR(T/A) (EIF4EBP1 Synthetic, Human)

Use

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

4EBP1 domain contains T37A and T46A mutations.

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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H11-mScarletSIIN

Plasmid

#172438

Purpose

Hipp11 targeting vector, expresses mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-SIIN

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mScarlet-MYO10

Plasmid

#145179

Purpose

Expresses mScarlet-MYO10 in mammalian cells

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

mScarlet-MYO10 (MYO10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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LMNA_mScarlet-I_Donor

Plasmid

#178092

Purpose

HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA-mScarlet-I HDR donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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VPS13D^mScarlet

Plasmid

#174110

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Use

Tags

mScarlet

Expression

Mammalian

Mutation

With an internal mScarlet tag in aminoacid positi…

Promoter

CMV

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET50_MDA5∆2CARD

Plasmid

#216804

Purpose

Expresses melanoma differentiation-associated protein 5 lacking its 2 CARD domains

Depositor

Sun Hur

Insert

melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)

Use

Tags

Expression

Bacterial

Mutation

∆1-287

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-LaminB

Plasmid

#98831

Purpose

In vivo visualization of the nuclear envelope (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

CytERM

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-mScarlet

Plasmid

#233210

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with mScarlet marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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mCarmine in pcDNA3

Plasmid

#109486

Purpose

mammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pOVC1_TtCarH-CBD_X

Plasmid

#131179

Purpose

Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

TtCarH-CBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_MxCarH-CBD_X

Plasmid

#131178

Purpose

Expresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

MxCarH-CBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-pHScarlet

Plasmid

#191258

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet

Depositor

Robert Campbell

Insert

pHScarlet

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-mScarlet

Plasmid

#140875

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCyterm-mScarlet3_N1

Plasmid

#189778

Purpose

Construct for testing oligimerization of mScarlet3 in mammalian cells by OSER assay

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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mito-mScarlet

Plasmid

#141153

Purpose

Fluorescent labeling of the mitochondrial matrix in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

COX4 (COX4 Budding Yeast)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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