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pAc5.1B-lambdaN-HA-DmLARK_N

Plasmid

#146981

Purpose

Insect Expression of DmLARK

Depositor

Elisa Izaurralde

Insert

DmLARK (lark Fly)

Expression

Insect

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLarp_N

Plasmid

#146982

Purpose

Insect Expression of DmLarp

Depositor

Elisa Izaurralde

Insert

DmLarp (larp Fly)

Expression

Insect

Mutation

two non silent mutation N110T, P173L compared to …

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2

Plasmid

#116999

Purpose

Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Rivularia sp. PCC 7116 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC

Plasmid

#117000

Purpose

Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Rivularia sp. PCC 7116 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC

Plasmid

#116998

Purpose

Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Rivularia sp. PCC 7116 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Fibrilarin/pcDNA3

Plasmid

#67781

Purpose

Fibrilarin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor-Fibrilarin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Phamret-Fibrillarin/pcDNA3

Plasmid

#51954

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localization

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Tags

Fibrillarin

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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FCK-CaMKII-Solaris-WPRE

Plasmid

#217480

Purpose

Negative response-polarity hybrid voltage indicator

Depositor

Peng Zou

Insert

Solaris

Use

Lentiviral

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pTriEx-RhoA FLARE.sc Biosensor WT

Plasmid

#12150

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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LRP4 (extracellular) scFv [N207/27]

Plasmid

#190537

Purpose

Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-ADAM22 (extracellular) [N57/2R]

Plasmid

#177564

Purpose

Mammalian Expression Plasmid of anti-ADAM22 (extracellular) (Mouse). Derived from hybridoma N57/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ADAM22 (extracellular) (Mus musculus) recombinant mouse monoclonal antibody (Adam22 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Lari-BFP2-TSERex

Plasmid

#123311

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Lari-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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