We narrowed to 7,375 results for: ef1a

1. pAAV-pCaMKII-tTAc

   Plasmid

   #172125

   Purpose

   Expression of InteinC-tTAC in CaMKII positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   pCaMKII, tTAc
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_831

   Plasmid

   #216100

   Purpose

   CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIA3-Olônne

   Plasmid

   #214447

   Purpose

   Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH231-EFS-Cas9-BlastR

   Plasmid

   #115146

   Purpose

   Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Cas9-T2A-BlastR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

   Plasmid

   #197893

   Purpose

   Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   EF1a

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMV_AA088

   Plasmid

   #216104

   Purpose

   Knockout, EF1a-driven Cas9 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

   Plasmid

   #178575

   Purpose

   Lentiviral vector for GFAP::Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CRY2PHR-P2A-CIBN-mCherry-VAMP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   GFAP

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-SAM-hygro

   Plasmid

   #138955

   Purpose

   Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

   Depositor

   Nadya Dimitrova

   Article

   Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 AND EF1a

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMV_AA090

   Plasmid

   #216099

   Purpose

   Knockout, EF1a-driven Cas12a (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV V5-LAP2beta

    Plasmid

    #175112

    Purpose

    Lentiviral expression of V5-tagged mouse Tmpo

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmpo (Tmpo Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

    Plasmid

    #201560

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pIND(SP1)

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWPXL-Hes1

    Plasmid

    #36983

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Hes1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA065

    Plasmid

    #215965

    Purpose

    Fragmid fragment: (Pol 2 promoter) human EF1a promoter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    EF1a_v1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH231-EF1-BLST-Cas9-VPR

    Plasmid

    #115147

    Purpose

    Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

    Depositor

    Michael Phelps, Raymond Monnat

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    BlastR-P2A-Cas9-VPR
    

    Use

    CRISPR

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJL179

    Plasmid

    #243688

    Purpose

    Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1

    Depositor

    Jonathan Weissman

    Article

    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

    Insert

    UTRs and CDS of SUPV3L1 (SUPV3L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ10531

    Plasmid

    #239267

    Purpose

    Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    NLS-dPspCas13b-EGFP-ABI
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag tag and V5 tag

    Expression

    Mammalian

    Mutation

    H133A and H1058A in PspCas13b

    Promoter

    EF1a

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLV-mCherry-Parkin

    Plasmid

    #237397

    Purpose

    Lentivirus plasmid for the stable expression of mCherry-Parkin in mammalian cells

    Depositor

    James Hurley

    Article

    Rose et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

    Insert

    PRKN (PRKN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236376

    Purpose

    Overexpression of HyperdFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E184R, T279R, D340R, D409R

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236375

    Purpose

    Overexpression of dFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    dFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236363

    Purpose

    Overexpression of endAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    endAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E174R, S542R, K548R

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236362

    Purpose

    Overexpression of HyperdAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E174R, D256R, E321R, E371R

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236361

    Purpose

    Overexpression of dAsCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    dAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1_-DIO-Varnam1-WPRE

    Plasmid

    #240054

    Purpose

    Cre dependent Red fluorescent voltage indicator for mammalian expression

    Depositor

    Mark Schnitzer

    Article

    Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

    Insert

    VARNAM1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiGuideFE-mSG-Puro

    Plasmid

    #226522

    Purpose

    Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

    Depositor

    Neville Sanjana

    Article

    Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

    Insert

    hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    U6,EF1a

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-TET1-dCas9

    Plasmid

    #235599

    Purpose

    Expresses Tet1CD-dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xNLS and TET1 CD

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pEF.DEST51-PTPN11

    Plasmid

    #215523

    Purpose

    Expression vector with Shp2

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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