We narrowed to 4,633 results for: DUR

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pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR

Plasmid

#67931

Purpose

Translational reporter - ARHGAP5 UTRs

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

sfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase

Plasmid

#163404

Purpose

Expresses BMPR-2 lacking the kinase domain in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTon1(acgfp1)

Plasmid

#233648

Purpose

Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

Depositor

Takeo Kubo

Article

Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

Insert

target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'

Use

Amphibian expression, tet-on

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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Rec-PhoA/CsgA

Plasmid

#170787

Purpose

Recombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinase

Depositor

Urartu Seker

Article

Ahan et al Chembiochem. 2019 Jul 15;20(14):1799-180

Inserts

csgA (csgA )
phoA (phoA )

Use

Synthetic Biology

Tags

HHHHHH

Expression

Bacterial

Promoter

Inverted proD with Bxb1recognition sites and Inve…

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(T17N)

Plasmid

#22784

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (T17N) (RAC1 A. victoria, Human)

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 T17N.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

Plasmid

#225433

Purpose

Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

4xCBS:mDFR:Rep/RepA:Tnos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pcDNA3

Plasmid

#206051

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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MSP1594_2x_NLS

Plasmid

#110625

Purpose

Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
None

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pB-bsd-Flag-CNP

Plasmid

#126608

Purpose

Expresses N-terminal Flag-tagged human CNP

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

2'3' cyclic nucleotide phosphodiesterase (CNP Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CAG

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-HIS-CBM-Xmod-DocIII

Plasmid

#91698

Purpose

CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-HIS-CBM-Xmod-DocIII

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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ins:MCS; cryaa:RFP

Plasmid

#110282

Purpose

A zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Ypet-PBD

Plasmid

#24533

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Use

Retroviral

Tags

Ypet

Expression

Mammalian

Available Since

Aug. 19, 2010

Availability

Academic Institutions and Nonprofits only
pLCHKO

Plasmid

#155048

Purpose

Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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PB_EF1a-CasRx-msfGFP-2A-Blast

Plasmid

#226010

Purpose

CasRx protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

CasRx RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pUB-nHyPer2 (NLS:HyPer2:NLS)

Plasmid

#84730

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS (nuclear localisation signal and NLS (nuclear…

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS5

Plasmid

#104354

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7_Dual_sgRNA

Plasmid

#173206

Purpose

Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

Dual U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1_V5_mNeonGreen_FLAG_C

Plasmid

#218073

Purpose

RESOLUTE cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG-mNeonGreen-V5

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-CyPet-Rac1

Plasmid

#24534

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (RAC1 Human)

Use

Retroviral

Tags

CyPet

Expression

Mammalian

Available Since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only