Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 30,244 results for: PLE

Showing: 1781 - 1800 of 30244 results

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pLenti6.2-coHPDL H258A 3xFlag-V5

Plasmid

#174130

Purpose

Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized, H258A

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-WT-puro

Plasmid

#156170

Purpose

Lentiviral vector for expression of RAP2A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX305-N-dTAG-CARD8-ZUC

Plasmid

#169990

Purpose

Mammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

dTAG

Expression

Mammalian

Promoter

hPGK

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

Plasmid

#160614

Purpose

TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

mini35s

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Mutation

T161A

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K227R-HA

Plasmid

#139859

Purpose

Lentiviral expression of human DDRGK1-K227R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K227R

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K121R-HA

Plasmid

#139852

Purpose

Lentiviral expression of the cDNA indicated

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K121R

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K116R-HA

Plasmid

#139850

Purpose

Lentiviral expression of human DDRGK1-K116R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K116R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K120R-HA

Plasmid

#139851

Purpose

Lentiviral expression of human DDRGK1-K120R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K120R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K124R-HA

Plasmid

#139853

Purpose

Lentiviral expression of human DDRGK1-K124R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K124R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K128R-HA

Plasmid

#139854

Purpose

Lentiviral expression of human DDRGK1-K128R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K128R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K146R-HA

Plasmid

#139855

Purpose

Lentiviral expression of human DDRGK1-K146R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K146R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K176R-HA

Plasmid

#139856

Purpose

Lentiviral expression of human DDRGK1-K176R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K176R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K193R-HA

Plasmid

#139857

Purpose

Lentiviral expression of the cDNA indicated

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K193R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K224R-HA

Plasmid

#139858

Purpose

Lentiviral expression of human DDRGK1-K224R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K224R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSHF1-P2A-Puro

Plasmid

#136901

Purpose

Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHF1

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

R661A,Q695A,Q926A

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-TMED3-Reverse-Complement

Plasmid

#136018

Purpose

The Reverse Complement of TMED3 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

TMED3 3'UTR (Reverse Complement) (TMED3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-ZC3H15-Reverse-Complement

Plasmid

#136019

Purpose

The Reverse Complement of ZC3H15 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

ZC3H15 3'UTR (Reverse Complement) (ZC3H15 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-TIRAP-cGASΔN-HA

Plasmid

#130921

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

TIRAP-ΔNcGAS

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the N ter…

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Fyn-cGASΔN-HA

Plasmid

#130922

Purpose

Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Fyn-ΔNcGAS

Tags

HA

Expression

Mammalian

Mutation

cGAS N terminus (aa1-159) replaced with the dual …

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 1781 - 1800 of 30244 results

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