We narrowed to 4,245 results for: Pet2;

1. pET28b-NmCas9-Flag D16A+H588A

   Plasmid

   #109033

   Purpose

   D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Use

   Tags

   Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Hook3 I136A, I139A, M142A

   Plasmid

   #74617

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b(+)_S. cerevisiae Hbs1(166-611)

   Plasmid

   #39338

   Depositor

   SeWon Suh

   Insert

   Elongation factor 1 alpha-like protein
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-ybbR-HIS-10FNIII(x4)-DocI

   Plasmid

   #58712

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   10FNIII(x4)-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-His6-GFP-UROD-N367A

   Plasmid

   #236742

   Purpose

   Protein overexpression of eGFP-human uroporphyrinogen decarboxylase N367A

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   eGFP-Uroporphyrinogen decarboxylase human N367A (UROD Human, Synthetic)
   

   Use

   Tags

   His6 and eGFP

   Expression

   Bacterial

   Mutation

   N367A

   Promoter

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a asyn(Δ41-54/Δ103-130)

   Plasmid

   #242236

   Purpose

   Expresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 103-130) in bacteria

   Depositor

   Jennifer Lee

   Article

   SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

   Insert

   asyn(Δ41-54/Δ103-130)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   deleted amino acids 41-54 and 103-130

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a asyn(66-140) K96A/K97A

   Plasmid

   #240590

   Purpose

   Overexpress truncated alpha-Synuclein protein

   Depositor

   Jennifer Lee

   Article

   McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

   Insert

   aSyn (SNCA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 66-140 only, K96A, K97A

   Promoter

   T7

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21a asyn(66-140) E104A/E105A

   Plasmid

   #240589

   Purpose

   Overexpress truncated alpha-Synuclein protein

   Depositor

   Jennifer Lee

   Article

   McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

   Insert

   aSyn (SNCA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 66-140 only, E104A, E105A

   Promoter

   T7

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a asyn(66-140) E83K/K97E

   Plasmid

   #240591

   Purpose

   Overexpress truncated alpha-Synuclein protein

   Depositor

   Jennifer Lee

   Article

   McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

   Insert

   aSyn (SNCA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 66-140 only, E83K, K97E

   Promoter

   T7

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a asyn(66-140) K80E/E83K

    Plasmid

    #240593

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K80E

    Promoter

    T7

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a asyn(66-140) E83K/K96E

    Plasmid

    #240592

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K96E

    Promoter

    T7

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid

    #224697

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid

    #224698

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. ECMAB - pET22b Ag43 160N 6H Mms6

    Plasmid

    #225539

    Purpose

    Construction of extracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Autotransporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET22b Ag43 160N 6H mCherry-R5 AmpR

    Plasmid

    #225141

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 160N, mCherry
    

    Use

    Tags

    Ag43 protein fused to silaffin-R5 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28b-SUMO-FLAG-TeKaiA-FLAG

    Plasmid

    #229220

    Purpose

    For expression of T. elongatus KaiA protein

    Depositor

    Andy LiWang

    Insert

    kaiA (kaiA T. elongatus)
    

    Use

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-SUMO-FLAG-TeKaiB-FLAG

    Plasmid

    #229221

    Purpose

    For expression of T. elongatus KaiB protein

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB T. elongatus)
    

    Use

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28b-SUMO-SeKaiB-K25C-FLAG

    Plasmid

    #229225

    Purpose

    For expression of S. elongatus KaiB protein mutant

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB S. elongatus)
    

    Use

    Tags

    FLAG and SUMO

    Expression

    Bacterial

    Mutation

    K25C

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10

    Plasmid

    #226351

    Purpose

    Expression of Titin (V15P) substrate for 26S proteasome

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5

    Plasmid

    #226352

    Purpose

    Expression of Titin (V15P) substrate for 26S proteasome

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V15P

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge