We narrowed to 4,933 results for: gne

1. pX330_Tuba1b sgRNA / hSpCas9

   Plasmid

   #172835

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Ro2

   Plasmid

   #24503

   Depositor

   Bruce Conklin

   Article

   Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

   Insert

   RASSL Ro2 (OPRK1 Human)
   

   Use

   Tags

   FLAG and NLS from prolactin

   Expression

   Mammalian

   Mutation

   mutations in extracellular loops 2 (see comments …

   Promoter

   Available since

   June 16, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pCRII-Sema4a-ISH-2kb

   Plasmid

   #68029

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Semaphorin-4A (Sema4a Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   a 2kb cDNA fragment for ISH probe synthesis

   Promoter

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. UAS-u(XL)Cas9

   Plasmid

   #127386

   Purpose

   Expresses Cas9 at very low level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(XL)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT2-4xUAS:P2A-mRFP

   Plasmid

   #127593

   Purpose

   This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenCMV-PMMars1CyAVPR2

   Plasmid

   #75226

   Purpose

   Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2

   Depositor

   Alan Waggoner

   Article

   Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

   Insert

   Mars1Cy-AVPR2 (AVPR2 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScalps_puro_mklf1

   Plasmid

   #59918

   Purpose

   Expresses mouse Kruppel-like factor1

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   Kruppel-like factor 1 (Klf1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. TagBFP(S2h)3(S4h)3-gs-P6f

    Plasmid

    #228083

    Purpose

    Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells.

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    BFP, S2h, S4h, P6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE30-DARPin-E11

    Plasmid

    #226784

    Purpose

    Expression of His-tagged DARPin E11 in E. coli

    Depositor

    Sebastian Mathea

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    DARPin E11
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas3-Apr

    Plasmid

    #208000

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. VF-Sensor

    Plasmid

    #208927

    Purpose

    in vitro RSK-Sensor for luminescent detection of RSK-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    SOS1 (SOS1 Human)
    

    Use

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PDE4B-Sensor

    Plasmid

    #208929

    Purpose

    in vitro JNK-Sensor for luminescent detection of JNK-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
    

    Use

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-DIO-myr-rsEGFP2- LDLR

    Plasmid

    #213399

    Purpose

    double floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2

    Depositor

    Katrin Willig

    Article

    Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

    Insert

    rsEGFP2
    

    Use

    AAV

    Tags

    C-terminal (Ct) cytoplasmic domains of low densit…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

    Plasmid

    #189634

    Purpose

    Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 10X PRE TK luc

    Plasmid

    #206162

    Purpose

    Luciferase reporter construct containing 10 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    10X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 8X PRE TK luc

    Plasmid

    #206161

    Purpose

    Luciferase reporter construct containing 8 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    8X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6X PRE TK luc

    Plasmid

    #206160

    Purpose

    Luciferase reporter construct containing 6 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    6X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    min TK

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 sf:em-blue-shift.1

    Plasmid

    #191928

    Purpose

    Encodes a GFP design, sf:em-blue-shift.1

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:em-blue-shift.1
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69P S72A T108V Y145M T167V V224I

    Promoter

    T7

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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