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We narrowed to 10,852 results for: kars;

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p3XP3-WhiteA2

Plasmid

#165875

Purpose

Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-Pidd1(T788D)

Plasmid

#60530

Purpose

Retroviral expression of Pidd1 T788D phosphorylation mutant

Depositor

Trudy Oliver

Article

Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

Insert

Pidd1 (PIDD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Threonine 788 to Aspartic acid*

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-mH6-Cas12i2

Plasmid

#120883

Purpose

Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12i2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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GFP/WT SP M85

Plasmid

#89317

Purpose

Expression of mouse GFP/WT spastin M85 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2008 Feb 27;28(9):2147-57. d

Insert

SPAST M85 (Spast Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pET11a

Plasmid

#71377

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pMOD6-pGRR-r11-r10-yeGFP

Plasmid

#74368

Purpose

pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

yeGFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

pGRR-r11-r10

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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3xFRB-myc

Plasmid

#20226

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Use

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
gRNA-mCtnnb1

Plasmid

#84542

Purpose

mCtnnb1 gRNA expressing vector

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mCtnnb1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H1_2

Plasmid

#36343

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pCfB9337 (pgRNA_IV-1_NatMX)

Plasmid

#161590

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCK671.306

Plasmid

#192641

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-TK

Plasmid

#165881

Purpose

Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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BC-A1-002

Plasmid

#78689

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

Plasmid

#21800

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Use

Tags

GST and TEV

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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SP_H6_Halo_K73T_KDEL_pBABEpu

Plasmid

#183689

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Use

Retroviral

Tags

Expression

Mutation

K73T in Halotag

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPHAGE2-Myc-mTZAP

Plasmid

#87188

Purpose

lentiviral stable expression of Myc-TZAP (mouse) in mouse embryonic stem cells

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (Zbtb48 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

unknown

Available since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pQP-2460

Plasmid

#138973

Purpose

Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata1

Plasmid

#41085

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata1 (Gata1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pTRF.1-mCMV-udsVenus

Plasmid

#58518

Purpose

Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pB194

Plasmid

#61154

Purpose

Expression of KIN28 under GAL1 promoter in yeast

Depositor

Charles Brenner

Article

Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

Insert

TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221-OSF-TRIM5-21R

Plasmid

#79063

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5-21R

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

T7

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTH760-CEN-mCherry_v3

Plasmid

#40598

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Use

Tags

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRoquin1_1-509_AE

Plasmid

#148612

Purpose

Mammalian Expression of HsRoquin1_1-509

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1_1-509 (RC3H1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRoquin1_510-1133_AE

Plasmid

#148613

Purpose

Mammalian Expression of HsRoquin1_510-1133

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1_510-1133 (RC3H1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD8A-RGR-r10

Plasmid

#74370

Purpose

This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

RGR-r10

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G08

Plasmid

#67142

Purpose

Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2T SHP1 CS

Plasmid

#8595

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C453S (catalytically inactive)

Promoter

Available since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
pAC-U63-tgRNA-nlsBFP

Plasmid

#169029

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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