We narrowed to 7,032 results for: Pet2

1. pET29b-clbH-C-A2-PCP S1524A

   Plasmid

   #114158

   Purpose

   Expresses ClbH-C-A2-PCP S1524A

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Tags

   S-Tag, Thrombin site

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH with S…

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-ecDHFR-dCys-T46C

   Plasmid

   #110543

   Purpose

   Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   T46 mutated to C46, C85 mutated to A85, C152 muta…

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-ecDHFR-dCys-L54C

   Plasmid

   #110542

   Purpose

   Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   L54 mutated to C54, C85 mutated to A85, C152 muta…

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-ecDHFR-D27S/Y100F

   Plasmid

   #110827

   Purpose

   Bacterial expression of E.coli DHFR D27S/Y100F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   D27 mutated to S27, Y100 mutated to F100

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-NmCas9-Flag D16A

   Plasmid

   #109031

   Purpose

   D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. csd2_121-308,S166A/pET28b(+)

   Plasmid

   #83301

   Purpose

   csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1544
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-120, and changed Serine 166 …

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. csd2_121-308,T151A/pET28b(+)

   Plasmid

   #83300

   Purpose

   csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1544
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-120, and changed Threonine 1…

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. csd2_121-308,Y197F/pET28b(+)

   Plasmid

   #83299

   Purpose

   csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1544
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-120, and changed Tyrosine 19…

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. csd2_121-308,E165A/pET28b(+)

    Plasmid

    #83298

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Glutamic ac…

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Hook3 D102A, E108A

    Plasmid

    #74618

    Purpose

    Expresses a double mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-Chaetomium thermophilum LIC

    Plasmid

    #74782

    Purpose

    encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Chaetomium thermophilum dynein light intermediate chain
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. csd6_17-330/pET28b(+) N-tag

    Plasmid

    #71353

    Purpose

    csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

14. csd6_4-330/pET28b(+) N-tag

    Plasmid

    #71351

    Purpose

    csd6, construct:4-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

15. csd6_13-330/pET28b(+) N-tag

    Plasmid

    #71352

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

16. csd6_13-330_E110A/pET28b(+) N-tag

    Plasmid

    #71354

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Glutamic acid 110 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

17. csd6_13-330_Y133A/pET28b(+) N-tag

    Plasmid

    #71356

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tyrosine 133 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

18. csd6_13-330_C176A/pET28b(+) N-tag

    Plasmid

    #71360

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Cysteine 176 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

19. csd6_13-330_H160A/pET28b(+) N-tag

    Plasmid

    #71359

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 160 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. csd6_13-330_W158A/pET28b(+) N-tag

    Plasmid

    #71358

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tryptophan 158 to Alanine)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only