We narrowed to 7,645 results for: Ski;
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Plasmid#113686PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.DepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Cre/LoxTagsKRABExpressionMutationPromoterAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA
Plasmid#113685PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.DepositorInsertinverted de-catalyzed SaCas9
UseAAV, CRISPR, Cre/Lox, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614G-trunc
Plasmid#158077PurposeExpresses SARS-CoV-2 spike protein with a D614G truncated mutationDepositorAvailable SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTarget_AT1R-tTA
Plasmid#222851PurposeMammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activatorDepositorAvailable SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1
Plasmid#135422PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro
Plasmid#120560PurposeExpresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus productionDepositorInsertHRAS (HRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationG12VPromoterCMVAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1s
Plasmid#135420PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variantDepositorHas ServiceAAV1InsertjYCaMP1s
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614-trunc
Plasmid#158076PurposeExpresses SARS-CoV-2 spike protein with a truncationDepositorAvailable SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82701PurposeAAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertH1/TO gRNA Expression Cassette Containing Tet2 gRNA
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianMutationPromoterAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLD1
Plasmid#160805PurposeExpress POLD1DepositorInsertPOLD1 (POLD1 Human)
UseRetroviralTagsExpressionMutationCodon optimized C-terminal 230 amino acidsPromoterAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-ALDOA_sgRNA2
Plasmid#201591PurposeCRISPR/Cas9-mediated gene knock-outDepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16-HSF1 WT
Plasmid#71726Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RDDepositorInsertGal4 DBD HSF1 VP16 AD (HSF1 Herpes Simplex Virus protein VP16, Budding Yeast, Human)
UseTagsExpressionMammalianMutationPromoterAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA
Plasmid#113697PurposeU6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-Lyn-SH2-AviTag
Plasmid#214207PurposeBacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorAvailable SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-TEV-Grb2-SH2-mCherry-AviTag
Plasmid#214231PurposeBacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.DepositorInsertGrb2 SH2 domain (GRB2 Human)
UseTags6xHis, AviTag, and mCherryExpressionBacterialMutationPromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-Fyn-SH2-AviTag
Plasmid#214202PurposeBacterial expression of SH2 domain of the Fyn kinase (B- iso) with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertFYN kinase (FYN Human)
UseTags6xHis, AviTag, and SUMOExpressionBacterialMutationPromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-Lck-SH2-AviTag
Plasmid#214203PurposeBacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertLck kinase SH2 domain (LCK Human)
UseTags6xHis, AviTag, and SUMOExpressionBacterialMutationPromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-Fgr-SH2-AviTag
Plasmid#214205PurposeBacterial expression of SH2 domain of the Fgr kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertFgr kinase SH2 domain (FGR Human)
UseTags6xHis, AviTag, and SUMOExpressionBacterialMutationPromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-Hck-SH2-AviTag
Plasmid#214206PurposeBacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertp60-HCK (HCK Human)
UseTags6xHis, AviTag, and SUMOExpressionBacterialMutationPromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-TAZ-CAMTA1
Plasmid#235679PurposeExpress TAZ-CAMTA1 fusion gene in mammalian cells using the CMV promoterDepositorAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only