We narrowed to 11,729 results for: dna plasmids
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pcDNA3.1+-Tornado-nLuc
Plasmid#212611PurposeFor expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.DepositorInsertTornado-CVB3-nLuc
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Antares2 c-myc
Plasmid#100027PurposeA BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescenceDepositorInsertAntares2
Tagsc-mycExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_LuxSit_i_mTagBFP2
Plasmid#194300PurposeMammalian expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
TagsmTagBFP2ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1_mouseST6galI
Plasmid#178435Purposemurine St6gal1 expression plasmidDepositorInsertSt6gal1 (St6gal1 Mouse)
Tags6xHIS-TEV Cleavage SiteExpressionMammalianMutationCoding sequnce for amino acids 85-403PromoterCMVAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-NL63-D14
Plasmid#172666Purposeencodes NL63 spike protein, for pseudovirus productionDepositorInsertHCNV63gp2 (HCNV63gp2 human coronavirus NL63)
UseLentiviral and RetroviralTagsNAExpressionMammalianMutationc-terminal deletion of 14aaPromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRBPJ.1.0-gDNA
Plasmid#132474PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertRBPJ (RBPJ Human)
UseCRISPRAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
c-Flag pcDNA3
Plasmid#20011PurposeThis pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsFlagExpressionMammalianAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Pu.1
Plasmid#66974PurposeExpression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of proteinDepositorAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CCR5
Plasmid#98943PurposeMammalian expression plasmid for untagged human CCR5DepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_NSlmb-vhhGFP4
Plasmid#35579DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionMammalianAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(-)2G
Plasmid#62799PurposemtZFN2G architecture bearing (-) FokI domainDepositorInsertmtZFN(-)2G
TagsInternal FLAG tag immediately after F1B mitochond…ExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(+)2G
Plasmid#62798PurposemtZFN2G architecture bearing (+) FokI domainDepositorInsertmtZFN(+)2G
TagsInternal HA tag immediately after F1B mitochondri…ExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt
Plasmid#157702Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
TagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT
Plasmid#218460PurposeMammalian expression plasmid for SARS-CoV-2 spike (1273 residues)DepositorInsertSARS-CoV-2 spike protein (1273 residues)-wild type (S )
ExpressionMammalianAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-spikeRBD::∆ACE2 SURF
Plasmid#206957PurposeFluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2DepositorTagscSURF, mCherry, and nSURFExpressionMammalianMutation17-615 and RBD (319-541)PromoterCMVAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG
Plasmid#176060PurposeExpresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.DepositorInsertCoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
Tags3xFLAGExpressionMammalianMutationK164A and H165APromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG
Plasmid#176059PurposeExpressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.DepositorInsertCoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
Tags3xFLAGExpressionMammalianMutationR124A and K125APromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu
Plasmid#218461PurposeMammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)DepositorInsertSARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
ExpressionMammalianMutationThr1273AGlu substitutionAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp
Plasmid#218462PurposeMammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)DepositorInsertSARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
ExpressionMammalianMutationThr1273Asp substitutionAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only
