We narrowed to 11,956 results for: nls

1. pPotef-LN22*-3xGNLS-cbx

   Plasmid

   #86780

   Purpose

   Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

   Depositor

   Michael Feldbrügge

   Article

   Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

   Insert

   LN*
   

   Use

   Ustilago maydis expression

   Tags

   Gfp

   Expression

   Bacterial

   Promoter

   Potef

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myog'-2A-mCherryNLS-PGK-Puro

   Plasmid

   #69548

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm
   Myogenin 3' homology arm (Myog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-NLS-gs10-cDHFR174

   Plasmid

   #188256

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cDHFR174 (DHFR Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. nDHFR173-gs10-NLS-VPR

   Plasmid

   #188262

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nDHFR173 (DHFR Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-FNLSHiFi-P2A-Puro

   Plasmid

   #136902

   Purpose

   Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFi
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Mutation

   R691A

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

   Plasmid

   #183803

   Purpose

   AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

   Insert

   E2-Crimson-NLS
   

   Use

   AAV

   Tags

   NLS

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC-U63-tgRNA-nlsBFP

   Plasmid

   #169029

   Purpose

   gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

   Depositor

   Chun Han

   Article

   Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Myog'-2A-mCherryNLS-PGK-Purodtk

   Plasmid

   #69547

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm (Myog Mouse)
   Myogenin 3' homology arm (Myog Mouse)
   Puro-delta-tk
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPD355 VPR-FRB (NLS)

   Plasmid

   #138847

   Purpose

   Constitutive expression of VPR-FRB under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-FRB
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2CG2-QUASM1:mScarletNLS-SV40pA

    Plasmid

    #155124

    Purpose

    Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1:mScarletNLS
    

    Use

    Zebrafish expression

    Promoter

    QUASM1-E1b

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPHR-mCherry-NLS-VP16

    Plasmid

    #103821

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-VP16 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPHR-iRFP713-NLS-VP16

    Plasmid

    #103823

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-iRFP713-VP16 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. ptagBFP-LacI-NLS-VP16

    Plasmid

    #103837

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-LacI-NLS-VP16
    

    Expression

    Mammalian

    Promoter

    SV2

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

    Plasmid

    #47453

    Purpose

    epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE (N136,Grm2,C63)-cib1
    

    Use

    AAV; Tale

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLR5-CAG-NLS-tdiRFP670

    Plasmid

    #82621

    Purpose

    Expresses NLS-tdiRFP670 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pattB-nsyb-MKII::nlsLexADBDo

    Plasmid

    #64725

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKII::nlsLexADBDo
    

    Expression

    Insect

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-MCS-YFP-NLS

    Plasmid

    #145274

    Purpose

    Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MCS and YFP
    

    Expression

    Mammalian

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2-7xTcf-NLS-ONL-CP

    Plasmid

    #65716

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2-7xTcf-NLS-YNL-CP

    Plasmid

    #65714

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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