We narrowed to 8,656 results for: pcDNA3

1. pcDNA3-N-HA-NEK7 K64M

   Plasmid

   #75143

   Purpose

   Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Lys 64 mutated to Met; kinase inactive and F236L …

   Promoter

   CMV

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyn-PlcR

   Plasmid

   #181830

   Purpose

   Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-PlcR
   

   Tags

   ECFP, EYFP, and N-terminal targeting sequence fro…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)_Granulin 4-no linker

   Plasmid

   #176925

   Purpose

   express Granulin 4 without linker 5 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 4 (GRN Human)
   

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Smac(1-60)mCherry

   Plasmid

   #40880

   Depositor

   Douglas Green

   Article

   Tait et al Dev Cell. 2010 May 18;18(5):802-13.

   Insert

   Smac(1-60)mCherry (DIABLO Human)
   

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-CaMPARI V398D

   Plasmid

   #60786

   Purpose

   Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Eric Schreiter, Loren Looger

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI V398D
   

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   V398D

   Promoter

   CMV

   Available Since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-GFP-p48

   Plasmid

   #11616

   Depositor

   Steven Johnson

   Insert

   Interferon-Stimulated Gene Factor 3 (IRF9 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.2-DEST-MatryoshCaMP6s

   Plasmid

   #100025

   Purpose

   Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Tags

   V5 Epitope Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/HisC-mTAZ

   Plasmid

   #31793

   Depositor

   Michael Yaffe

   Article

   Hong et al Science. 2005 Aug 12;309(5737):1074-8.

   Insert

   TAZ (Wwtr1 Mouse)
   

   Tags

   EK (Enterokinase) Recognition Site, His, and Xpre…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-HA-Akt1-K179M

   Plasmid

   #73409

   Purpose

   Expresses kinase-inactive mutant of Akt with HA tag

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed K179M to make Kinase inactive mutant

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 AKT3 HA (CT-gfp)

    Plasmid

    #27293

    Depositor

    Morris Birnbaum

    Insert

    protein kinase B gamma (Akt3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y473F

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 mouse IL-6 (1240)

    Plasmid

    #66908

    Purpose

    Mammalian expression of IL-6

    Depositor

    Michael Karin

    Insert

    IL-6 (Il6 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HA-mSTING (N153S)

    Plasmid

    #214158

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (N153S) (Sting1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N153S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-mTUT3

    Plasmid

    #60040

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT3

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT3 (Papd5 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-Lyso-InPAkt

    Plasmid

    #184053

    Purpose

    Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Lyso-InPAkt
    

    Tags

    Lysosome-associated membrane protein 1 (LAMP1).

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-MS2-HA-HsSmg5_T

    Plasmid

    #147552

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5 (SMG5 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. hOlig1-myc/pCDNA3.1

    Plasmid

    #197337

    Purpose

    Mammalian expression of an epitope-tagged hOLIG1

    Depositor

    Charles Stiles

    Insert

    OLIG1(pSM1-3) (OLIG1 Human)
    

    Tags

    Myc-his

    Expression

    Mammalian

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcdna3 flag - ULK2 wt

    Plasmid

    #27637

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse ULK2 (Ulk2 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Initial M removed from ULK2.

    Available Since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-RON-V5/HIS

    Plasmid

    #201983

    Purpose

    expression of human RON receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RON receptor tyrosine kinase (MST1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-pri-let-7b

    Plasmid

    #51378

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    pri-let-7b (MIRLET7B Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 c-SRC (1-249)

    Plasmid

    #42204

    Depositor

    Robert Lefkowitz

    Article

    Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

    Insert

    c-SRC (1-249) (SRC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains amino acids 1-249

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only