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pMIA4.721-Keppel

Plasmid

#214444

Purpose

BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Plasmid

#137830

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Leucine 71 to Lysine in CTEVp fragment

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-TNNT2-Neo

Plasmid

#214013

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Article

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-RPA2

Plasmid

#22893

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

RPA2 (RPA2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Tags

StrepII-Avi-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-AlaRS

Plasmid

#224362

Purpose

Express AlaRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

AlaRS (alaS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-Z4 (GB3375)

Plasmid

#160648

Purpose

pLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Olônne

Plasmid

#214443

Purpose

BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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