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SuExp-hPLD4

Plasmid

#201244

Purpose

express full length human PLD4 protein

Depositor

David Nemazee

Article

Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

Insert

PLD4 (PLD4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCI H2B-GFP

Plasmid

#92399

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

IRES H2B EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-VP64

Plasmid

#92363

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCI Cas9 H2B-RFP

Plasmid

#92397

Purpose

CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcU6_1 sgRNA

Plasmid

#92395

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.1 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.1

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcg 2.0-BFP-circ-arRNA backbone

Plasmid

#213965

Purpose

pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1mut-PA-mCherry

Plasmid

#138251

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-PAmCherry

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F2bbs kan GCANNNAGGk

Plasmid

#18752

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F3bbs kan NNNTGGGCGk

Plasmid

#18756

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F1bbs kan GCGTGGNNN

Plasmid

#18753

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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TDK promoter dCas9

Plasmid

#113657

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

TDK gene promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

TDK gene promoter

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-U6ac-Praf2-guides

Plasmid

#254765

Purpose

Praf2 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Praf2 (praf2 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Rabep1-1-guides

Plasmid

#254761

Purpose

Rabep1 gRNAs for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rabep1 (rabep1 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Arhgap20-guides

Plasmid

#254763

Purpose

Arhgap20 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Arhgap20 (arhgap20 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Vamp8-guides

Plasmid

#254764

Purpose

Vamp8 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Vamp8 (vamp8 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-APOBEC1-YTHmut-EGFP

Plasmid

#209323

Purpose

AAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1--YTHmut-HA

Use

AAV

Tags

HA

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EGFP-APOBEC1-YTHmut

Plasmid

#209320

Purpose

AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-YTHmut-HA

Use

AAV

Tags

HA

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-dt/deltaEGFP

Plasmid

#163919

Purpose

PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

dtomato

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1mut-Dronpa

Plasmid

#138250

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-Dronpa

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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