We narrowed to 14,142 results for: Cre

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pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

Plasmid

#107943

Purpose

Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain

Tags

3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

Expression

Mammalian

Mutation

E174R, S542R, K548R and D908A

Promoter

CAG

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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COVID-SARS2 NSP13

Plasmid

#159614

Purpose

Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

Depositor

Opher Gileadi

Article

Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

Insert

Covid-SARS2 Nsp13

Tags

His6-Zb-TEV

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

T7 - LacO

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBB535

Plasmid

#27392

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ (dnaJ, dnaK E. coli)

Expression

Bacterial

Available Since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

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reporter-gT1

Plasmid

#47320

Purpose

TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM33

Plasmid

#65398

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM33 (TRIM33 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-EmGFP-LATS2/1 KD

Plasmid

#52085

Purpose

Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter

Depositor

Yutaka Hata

Article

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

LATS2 and LATS1 miRNAs (LATS1, LATS2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-IL6

Plasmid

#111933

Purpose

Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Interleukin 6 (IL6 Human)

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Purpose

Expresses rM3D (Gs) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

rM3D (Gs)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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LentiGuide Blast

Plasmid

#199622

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

Depositor

Danwei Huangfu

Article

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

Plasmid

#203839

Purpose

Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mScarlet-KASH-2A

Mutation

Enzymatically inactive Cre

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB504

Plasmid

#74846

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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WFS1-Flag-pcDNA3

Plasmid

#13011

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pOPINM

Plasmid

#26044

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His-MBP-3C-lacZ

Tags

MAHHHHHHSSG_MBP_LEVLFQGP

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pLentiFUCCI(CA)5

Plasmid

#223176

Purpose

Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

Depositor

Neville Sanjana

Article

Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

Insert

FUCCI(CA)5

Use

Lentiviral

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKdm6a#2/Cre

Plasmid

#173638

Purpose

Expresses a Kdm6a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kdm6a (Kdm6a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPten#2/Cre

Plasmid

#173646

Purpose

Expresses a Pten-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pten (Pten Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKdm6a#1/Cre

Plasmid

#173637

Purpose

Expresses a Kdm6a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kdm6a (Kdm6a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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