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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,462 results for: GAN

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Showing: 1821 - 1840 of 7462 results

  1. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid
    #112924
    Purpose
    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker
    Depositor
    Kosuke Yusa
    Publication
    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    Use
    Piggybac transposon vector
    Expression
    Mammalian
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    112924-plasmid-map-sequence-id-217554

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  3. pAP118

    Plasmid
    #105495
    Purpose
    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions
    Depositor
    Wiep Klaas Smits
    Publication
    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HupA-SmBiT/HupA-LgBiT
    Use
    Atc-dependent expression in c. difficile
    Promoter
    Ptet
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105495-plasmid-map-sequence-id-209872

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  5. gCOTS-pyl

    Plasmid
    #92048
    Purpose
    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.
    Depositor
    Lital Alfonta
    Publication
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Cyanobacteria s. elongatus genome recombination i…
    Promoter
    LeuP (native S. elongatus promoter) and PrcbL
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92048-plasmid-map-sequence-id-181707

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  6. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid
    #197265
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Publication
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197265-plasmid-map-sequence-id-389723

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  8. pCG-TPMV Hd32-IL-13

    Plasmid
    #163869
    Purpose
    Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells
    Depositor
    Jakob Reiser
    Publication
    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPMV H glycoprotein/IL-13
    Tags
    Human IL-13
    Expression
    Mammalian
    Mutation
    Amino acids 2 to 33 at N terminus deleted
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163869-plasmid-map-sequence-id-325508

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  9. pUBC_HA_PP7-mVenus

    Plasmid
    #111268
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-mVenus
    Use
    Synthetic Biology
    Tags
    HA, SV40 NLS, and mVenus
    Expression
    Mammalian
    Mutation
    PP7: C68S, S69G [delta]70-75 (non-aggregating)
    Promoter
    hUBC
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    111268-plasmid-map-sequence-id-212603

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  11. MMP11-bio-His

    Plasmid
    #53408
    Purpose
    Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    MMP11 (MMP11 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53408-plasmid-map-sequence-id-83189

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  12. PDIA5-bio-His

    Plasmid
    #52072
    Purpose
    Expresses full-length Protein disulfide-isomerase A5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PDIA5 (PDIA5 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52072-plasmid-map-sequence-id-80297

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  14. pGL3-NBRE3

    Plasmid
    #208254
    Purpose
    pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells
    Depositor
    Douglas Kojetin
    Publication
    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3X NBRE-LUC
    Expression
    Mammalian
    Promoter
    SV40 early promoter
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    208254-plasmid-map-sequence-id-417965

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  17. pETM33_Nsp4

    Plasmid
    #156474
    Purpose
    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Publication
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp4 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156474-plasmid-map-sequence-id-307734

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  18. ICAM5-bio-His

    Plasmid
    #51759
    Purpose
    Expresses full-length Intercellular adhesion molecule 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ICAM5 (ICAM5 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51759-plasmid-map-sequence-id-79348

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Showing: 1821 - 1840 of 7462 results