We narrowed to 2,195 results for: IPP

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pCS2-OXER1-mKate2

Plasmid

#226713

Purpose

overexpression red fluorescent OXER1 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

OXER1 (OXER1 Human)

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHB1

Plasmid

#204668

Purpose

pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

Depositor

Mark Mandel

Article

Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

Insert

FRT-erm-FRT

Use

Synthetic Biology

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E

Plasmid

#125797

Purpose

Histone H2B-superfolder GFP and tdTomato fused by unskippable linker

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

H2BsfGFP-tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pOZ-FH-C Bach1 K141A/K142A

Plasmid

#73646

Purpose

Retroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Retroviral

Tags

FLAG and His

Expression

Mammalian

Mutation

K141A/K142A ( mlh1 binding mutant)

Promoter

unknown

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only
MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFH2.12_GagZip-MCP-P2A-eGFP

Plasmid

#205527

Purpose

Express HIV GagZip-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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DR274-eGFP sgRNA

Plasmid

#61051

Purpose

for generation of a eGFP specific sgRNA from a T7 promoter

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Insert

EGFP sgRNA

Use

CRISPR

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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GAE teo-GFP

Plasmid

#83958

Purpose

Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

TetO-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFH2.13_GagZip-MCP_Zip-P2A-eGFP

Plasmid

#205528

Purpose

Express HIV GagZip-MCP_Zip-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP_Zip

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEB2-mKate2

Plasmid

#104009

Purpose

Plasmid encoding mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mKate2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, V45A, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCB7 alpha5

Plasmid

#16041

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

alpha5 (ITGA5 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pCS2+cPLA2-mKate2

Plasmid

#162068

Purpose

Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Avian, xenopus, zebrafish

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pPIPL12-FnCas12a

Plasmid

#199563

Purpose

Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

Fncas12a

Use

CRISPR and Synthetic Biology; Tetracycline induci…

Expression

Bacterial

Promoter

COtetR-PIPL12

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mCherry

Plasmid

#99265

Purpose

Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

mCherry

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 beta1

Plasmid

#16042

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

beta1 (ITGB1 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pEB2-TagRFP-T

Plasmid

#103998

Purpose

Plasmid encoding TagRFP-T

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP-T

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

Plasmid

#69546

Purpose

Tet-On system expressing MyoD-2A-GFP-2xNLS.

Depositor

Charles Gersbach

Inserts

eGFP
Myod1 (Myod1 Mouse)

Use

Lentiviral

Tags

2x nuclear localization sequence and T2A "sk…

Mutation

Removed stop codon to fuse it to the 2A-eGFPnls i…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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