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Showing: 1821 - 1840 of 2873 results

  1. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91130-plasmid-map-sequence-id-182254

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  3. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37276-plasmid-map-sequence-id-46873

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  4. pDIRECT_26G

    Plasmid
    #91149
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91149-plasmid-map-sequence-id-182283

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  10. pHL 1158

    Plasmid
    #53007
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pMOD_B2203

    Plasmid
    #91066
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91066-plasmid-map-sequence-id-181864

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  13. pMOD_B2112

    Plasmid
    #91064
    Purpose
    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    PvUbi1
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91064-plasmid-map-sequence-id-181860

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  15. pMOD_B2101

    Plasmid
    #91060
    Purpose
    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    35S
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91060-plasmid-map-sequence-id-181852

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  17. pMOD_B2103b

    Plasmid
    #91062
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91062-plasmid-map-sequence-id-181865

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  18. pMOD_C2518

    Plasmid
    #91087
    Purpose
    Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    TaU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91087-plasmid-map-sequence-id-182052

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  19. pDIRECT_26H

    Plasmid
    #91150
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91150-plasmid-map-sequence-id-182284

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  20. pMOD_B2301

    Plasmid
    #91067
    Purpose
    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    35S
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91067-plasmid-map-sequence-id-181873

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Showing: 1821 - 1840 of 2873 results