We narrowed to 3,360 results for: actin

Showing: 1821 - 1840 of 3360 results

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pAC-luc-CLK

Plasmid

#33132

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

Insert

CLK 3' UTR (Clk Fly)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Insect

Promoter

Actin 5C

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCA-G-intron(Neo)-T(LNL)

Plasmid

#36907

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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tdEos-VASP-5

Plasmid

#57685

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VASP

Tags

tdEos

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV hGRN2

Plasmid

#213683

Purpose

Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

human Granulin-2

Use

AAV

Tags

twin-Strep-FLAG

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-lifeact-oROS-HT

Plasmid

#216420

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

lifeact-oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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Combined γB2FL-FRET probe

Plasmid

#209094

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Combined γB2ΔICD-FRET probe

Plasmid

#209093

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

Plasmid

#168254

Purpose

"label stable F-actin under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

Plasmid

#136912

Purpose

DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

pCAG-DmrC-NLS-3xFLAG-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE_NFL linker(A6TAG)-3xFLAG KI

Plasmid

#182680

Purpose

Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)

Expression

Mammalian

Promoter

U6 and chicken beta-actin promoter

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-CR-PA

Plasmid

#175445

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

BiP Secretion Peptide and Thrombin-Protein A

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

Plasmid

#168253

Purpose

"label stable F-actin; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-EGFP

Plasmid

#132974

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

eGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MultiMate-Rainbow

Plasmid

#206252

Purpose

Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)

Use

Recombinant baculovirus production (bac-to-bac)

Tags

iRFP713

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.1

Plasmid

#176544

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.1
Cas12f-GE ver4.1

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2s-CCR5TC

Plasmid

#231785

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2s-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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