We narrowed to 5,083 results for: mcs

1. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Promoter

   TET-On

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-TRIM28deltaBCC

   Plasmid

   #124954

   Purpose

   Mammalian expression of Human TRIM28 with deleted Bbox and CC domains

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of Bbox and CC domains

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLP701

   Plasmid

   #203743

   Purpose

   C-terminal tagging of target proteins with 3xV5-IAA17 degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xV5

   Promoter

   none

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_ER-RE_MLPmin_BC1065-luc2

   Plasmid

   #227111

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3413 pBF339 Bcl-XL

   Plasmid

   #8773

   Depositor

   Stanley Korsmeyer

   Article

   Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

   Insert

   Bcl-XL (Bcl2l1 Mouse)
   

   Expression

   Yeast

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pXPR-SF61

   Plasmid

   #163916

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277

   Insert

   SF5Phe tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-uTEV1-NES

   Plasmid

   #171006

   Purpose

   HiLITR protease with C-terminal NNES (Cytoplasmic)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-NES
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_ERBB2-V5

   Plasmid

   #214612

   Purpose

   ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   ERBB2 (ERBB2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA_SZ1-RGG-GFP-RGG

   Plasmid

   #124932

   Purpose

   Expression of SZ1-RGG-GFP-RGG

   Depositor

   Matthew Good, Benjamin Schuster, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-TSV (linearized in LT)

    Plasmid

    #70033

    Purpose

    pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region

    Depositor

    Mariet Feltkamp

    Article

    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

    Insert

    TSPyV full genome, linearized in Large T-antigen
    

    Use

    Cloning vector

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. G1333 DddAtox-N–dSpCas9

    Plasmid

    #157833

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST

    Plasmid

    #194386

    Purpose

    Gateway Destination vector for GPCR split TEV assays with V2R tail

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNP1 eforRed toehold switch

    Plasmid

    #107353

    Purpose

    Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 1 + eforRed
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-hPMCA2x/b

    Plasmid

    #47585

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    pMCA2x/b (ATP2B2 Human)
    

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA_RGG-RFP-RGG

    Plasmid

    #124934

    Purpose

    Expression of RGG-RFP-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENeGFP PCNAL2 pc653

    Plasmid

    #167564

    Purpose

    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

    Depositor

    Cristina Cardoso

    Article

    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV5-rat ERK2-K52R

    Plasmid

    #40813

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    K52R

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

19. EGFP-uTEV1-MAVS

    Plasmid

    #170998

    Purpose

    HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3537 pMIG Bim

    Plasmid

    #8786

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bim (Bcl2l11 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only